Qiu Yanjun, Jiang Hong, Dong Yueyang, Wang Yongzhen, Hamouda Hamed I, Balah Mohamed A, Mao Xiangzhao
College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China.
Processes Design and Development Department, Egyptian Petroleum Research Institute, Cairo 11727, Egypt.
Foods. 2022 Apr 1;11(7):1025. doi: 10.3390/foods11071025.
Fucoidan is one of the main polysaccharides of brown algae and echinoderm, which has nutritional and pharmacological functions. Due to the low molecular weight and exposure of more sulfate groups, oligo-fucoidan or fucoidan oligosaccharides have potential for broader applications. In this research, a novel endo-α-1,4-L-fucoidanase OUC-FaFcn1 which can degrade fucoidan into oligo-fucoidan was discovered from the fucoidan-digesting strain 12,076. OUC-FaFcn1 belongs to glycoside hydrolases (GH) family 107 and shows highest activity at 40 °C and pH 9.0. It can degrade the α-1,4 glycosidic bond, instead of α-1,3 glycosidic bond, of the fucoidan with a random tangent way to generate the principal product of disaccharide, which accounts for 49.4% of the total products. Therefore, OUC-FaFcn1 is a promising bio-catalyst for the preparation of fucoidan-derived disaccharide. These results further enrich the resource library of fucoidanase and provide the basis for the directional preparation of fucoidan-derived oligosaccharide with specific polymerization.
岩藻聚糖硫酸酯是褐藻和棘皮动物的主要多糖之一，具有营养和药理功能。由于分子量低且更多硫酸基团暴露，低聚岩藻聚糖硫酸酯或岩藻聚糖硫酸酯寡糖具有更广泛的应用潜力。在本研究中，从能够消化岩藻聚糖硫酸酯的菌株12,076中发现了一种新型的内切-α-1,4-L-岩藻聚糖硫酸酯酶OUC-FaFcn1，它可以将岩藻聚糖硫酸酯降解为低聚岩藻聚糖硫酸酯。OUC-FaFcn1属于糖苷水解酶（GH）家族107，在40℃和pH 9.0时表现出最高活性。它可以以随机切割的方式降解岩藻聚糖硫酸酯的α-1,4糖苷键，而不是α-1,3糖苷键，产生占总产物49.4%的主要产物二糖。因此，OUC-FaFcn1是一种有前途的生物催化剂，可用于制备岩藻聚糖硫酸酯衍生的二糖。这些结果进一步丰富了岩藻聚糖硫酸酯酶的资源库，并为定向制备具有特定聚合度的岩藻聚糖硫酸酯衍生寡糖提供了依据。
