R Shyamkrishnan, Saharia Gautom K, Panda Sandip, Mangaraj Manaswini
Biochemistry, All India Institute of Medical Sciences, Bhubaneswar, Bhubaneswar, IND.
Nephrology, All India Institute of Medical Sciences, Bhubaneswar, Bhubaneswar, IND.
Cureus. 2022 Mar 8;14(3):e22959. doi: 10.7759/cureus.22959. eCollection 2022 Mar.
Background Chronic kidney disease (CKD) involves a gradual loss of kidney function over months to years. Oxidative stress plays a critical role in the pathogenesis of CKD. Homocysteine (Hcy), an amino acid derivative, is a known risk factor for oxidative stress and endothelial damage. Gamma-glutamyl transferase (GGT), an enzyme abundant on the cell surface of liver and kidney cells, is raised during oxidative stress. The objectives of this study were to estimate the concentrations of serum Hcy and GGT among CKD patients and healthy controls and to determine whether there is an association between serum Hcy and GGT levels in CKD. Methodology A total of 246 participants were needed to meet the calculated sample size. A total of 123 CKD patients meeting the inclusion and exclusion criteria were recruited as cases from the Nephrology outpatient department of our institute. Equal numbers of age- and sex-matched healthy volunteers were recruited as controls. Biophysical profiling of participants was done. Baseline investigations were recorded. A blood sample was collected from each participant and analyzed for GGT and Hcy along with other routine parameters. Results Hcy and GGT concentrations were significantly high in CKD patients compared to healthy controls. There was a significant positive correlation between serum GGT and Hcy levels (r = 0.357). Conclusions Elevated levels of GGT and Hcy in CKD patients compared to healthy controls demonstrated the oxidative stress associated with the disease. GGT and Hcy can be used as prognostic markers of the disease.
背景 慢性肾脏病（CKD）是指在数月至数年的时间里肾功能逐渐丧失。氧化应激在CKD的发病机制中起关键作用。同型半胱氨酸（Hcy）是一种氨基酸衍生物，是已知的氧化应激和内皮损伤的危险因素。γ-谷氨酰转移酶（GGT）是一种在肝细胞和肾细胞表面大量存在的酶，在氧化应激期间会升高。本研究的目的是估计CKD患者和健康对照者血清Hcy和GGT的浓度，并确定CKD患者血清Hcy和GGT水平之间是否存在关联。方法 共需要246名参与者以满足计算出的样本量。从我院肾内科门诊招募了123名符合纳入和排除标准的CKD患者作为病例。招募了数量相等的年龄和性别匹配的健康志愿者作为对照。对参与者进行了生物物理特征分析。记录了基线检查情况。从每个参与者采集血样，分析GGT、Hcy以及其他常规参数。结果 与健康对照相比，CKD患者的Hcy和GGT浓度显著升高。血清GGT和Hcy水平之间存在显著正相关（r = 0.357）。结论 与健康对照相比，CKD患者的GGT和Hcy水平升高表明了与该疾病相关的氧化应激。GGT和Hcy可作为该疾病的预后标志物。
