Puttrich Verena, Rohlena Jakub, Braun Marcus, Lansky Zdenek
Institute of Biotechnology, Czech Academy of Sciences, BIOCEV, Prague West, Czech Republic.
Methods Mol Biol. 2022;2431:533-546. doi: 10.1007/978-1-0716-1990-2_28.
Intracellular trafficking of organelles driven by molecular motors underlies essential cellular processes. Mitochondria, the powerhouses of the cell, are one of the major cargoes of molecular motors. Efficient distribution of mitochondria ensures cellular fitness while defects in this process contribute to severe pathologies, such as neurodegenerative diseases. Reconstitution of the mitochondrial microtubule-based transport in vitro in a bottom-up approach provides a powerful tool to investigate the mitochondrial trafficking machinery in a controlled environment in the absence of complex intracellular interactions. In this chapter, we describe the procedures for achieving such reconstitution of mitochondrial transport.
由分子马达驱动的细胞器细胞内运输是基本细胞过程的基础。线粒体作为细胞的动力源，是分子马达的主要货物之一。线粒体的有效分布确保细胞健康，而这一过程中的缺陷会导致严重疾病，如神经退行性疾病。以自下而上的方式在体外重建基于微管的线粒体运输，为在没有复杂细胞内相互作用的可控环境中研究线粒体运输机制提供了一个强大的工具。在本章中，我们描述了实现这种线粒体运输重建的程序。
