School of Science, Jiangnan University, Lihu Avenue 1800, 214122 Wuxi, China; Jiangsu Provincial Research Center of Light Industrial Optoelectronic Engineering and Technology, Lihu Avenue 1800, 214122 Wuxi, China.
School of Science, Jiangnan University, Lihu Avenue 1800, 214122 Wuxi, China; Jiangsu Provincial Research Center of Light Industrial Optoelectronic Engineering and Technology, Lihu Avenue 1800, 214122 Wuxi, China; School of Internet of Things Engineering, Jiangnan University, Lihu Avenue 1800, 214122 Wuxi, China.
Spectrochim Acta A Mol Biomol Spectrosc. 2022 Aug 5;276:121212. doi: 10.1016/j.saa.2022.121212. Epub 2022 Mar 30.
The norfloxacin (NFX) residue in milk will increase human resistance to drugs and pose a threat to public health. In this work, a highly sensitive method for detection of NFX was developed based on surface enhanced Raman spectroscopy (SERS) using β-cyclodextrin functionalized silver nanoparticles (β-CD-AgNPs) as substrate. The unique spatial size and hydrophilicity of β-CD on the surface of AgNPs could selectively capture the target molecule (NFX) through some weak interactions, including hydrogen-bond interaction, electrostatic interaction, etc. The interactions were characterized by the UV-Vis absorption spectroscopy, fluorescence spectroscopy, Zeta potential and DLS. The Raman signal of NFX is largely enhanced when anchored by β-CD on the surface of AgNPs due to SERS effect. Through a series of experiments and analysis, the limit of detection (LOD) in standard solution and spiked milk were calculated to be 3.214 pmol/L and 5.327 nmol/L. The correlation coefficients (R) were 0.986 and 0.984, respectively. For milk sample determination of NFX, the recovery was 101.29% to 104.00% with the relative standard deviation (RSD) from 2.986% to 9.136%. To sum up, this developed SERS strategy is sensitive and specific to detect NFX in milk, it has practical application value and prospects.
牛奶中的诺氟沙星（NFX）残留会增加人体对药物的耐药性，对公众健康构成威胁。在这项工作中，我们基于表面增强拉曼光谱（SERS），使用β-环糊精功能化的银纳米粒子（β-CD-AgNPs）作为基底，开发了一种用于检测 NFX 的高灵敏度方法。β-CD 在 AgNPs 表面上独特的空间尺寸和亲水性可以通过一些弱相互作用（包括氢键相互作用、静电相互作用等）选择性地捕获目标分子（NFX）。通过紫外-可见吸收光谱、荧光光谱、Zeta 电位和 DLS 对相互作用进行了表征。由于 SERS 效应，NFX 的拉曼信号在被β-CD 锚定在 AgNPs 表面时得到了极大的增强。通过一系列实验和分析，计算了在标准溶液和牛奶加标中的检出限（LOD）分别为 3.214 pmol/L 和 5.327 nmol/L。相关系数（R）分别为 0.986 和 0.984。对于牛奶样品中 NFX 的测定，回收率为 101.29%至 104.00%，相对标准偏差（RSD）为 2.986%至 9.136%。总之，该开发的 SERS 策略对牛奶中的 NFX 具有灵敏和特异性的检测能力，具有实际应用价值和前景。
