Lazarini Josy Goldoni, Massarioli Adna Prado, Soares Jackeline Cintra, Nani Bruno Dias, Charo Nancy, Oliveira Douglas Souza, Camargo Lauren, Alvarez-Flores Miryam Paola, Batista Isabel de Fátima Correia, Chudzinski-Tavassi Ana Marisa, Alencar Severino Matias de, Franchin Marcelo, Rosalen Pedro Luiz
Department of Biosciences, Piracicaba Dental School, University of Campinas, Limeira Avenue, 901, Areião, 13414-903 Piracicaba, SP, Brazil.
Department of Agri-Food Industry, Food and Nutrition, "Luiz de Queiroz" College of Agriculture, University of São Paulo, Pádua Dias Avenue, P.O. Box. 9, 13418-900 Piracicaba, SP, Brazil.
Food Chem (Oxf). 2022 Mar 7;4:100093. doi: 10.1016/j.fochms.2022.100093. eCollection 2022 Jul 30.
We determined the phytochemical composition, anti-inflammatory mechanism of action, ROS/RNS scavenging capacity and systemic toxicity of a purified subfraction (S8) of . The composition of S8 was assessed by LC-ESI-QTOF-MS; the anti-inflammatory activity in RAW264.7 macrophages through NF-κB activation and biomarkers by multiplex in THP-1 cells; neutrophil migration, intravital microscopy and ICAM-1 expression in mice; NETs formation and CD11b expression; S8 scavenging capacity of ROS/RNS; toxicity in larvae model. Coumaric acid, quercetrin and vanillic acid were identified. S8 decreased NF-κB activation, IL-1β, IL-6, IL-10, MDC and MCP-1 levels, reduced neutrophil migration and ICAM-1 expression in mice; S8 did not interfere NET formation and CD11b expression, exhibited high antioxidant and showed negligible toxicity. proved to be a promising, yet underexplored source of bioactive compounds, which can be useful employed in agribusiness and in the pharmaceutical and food industry to develop new products or human health supplies.
我们测定了[某种物质]纯化亚组分（S8）的植物化学成分、抗炎作用机制、活性氧/氮清除能力和全身毒性。通过液相色谱-电喷雾-四极杆飞行时间质谱（LC-ESI-QTOF-MS）评估S8的成分；通过RAW264.7巨噬细胞中核因子κB（NF-κB）激活以及THP-1细胞中多重生物标志物检测其抗炎活性；检测小鼠中性粒细胞迁移、活体显微镜观察和细胞间黏附分子-1（ICAM-1）表达；检测中性粒细胞胞外诱捕网（NETs）形成和CD11b表达；检测S8对活性氧/氮的清除能力；检测其对[某种]幼虫模型的毒性。鉴定出了香豆酸、槲皮苷和香草酸。S8降低了NF-κB激活、白细胞介素-1β（IL-1β）、白细胞介素-6（IL-6）、白细胞介素-10（IL-10）、巨噬细胞衍生趋化因子（MDC）和单核细胞趋化蛋白-1（MCP-1）水平，减少了小鼠中性粒细胞迁移和ICAM-1表达；S8不干扰NET形成和CD11b表达，具有高抗氧化性且毒性可忽略不计。[某种物质]被证明是一种有前景但尚未充分探索的生物活性化合物来源，可用于农业综合企业以及制药和食品工业，以开发新产品或人类健康用品。
