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BcERF3 的过表达增加了柴胡中柴胡皂苷的生物合成。

Overexpression of BcERF3 increases the biosynthesis of saikosaponins in Bupleurum chinense.

机构信息

Institute of Medicinal Plant Development (IMPLAD), Chinese Academy of Medical Sciences & Peking Union Medical College (Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education & National Engineering Laboratory for Breeding of Endangered Medicinal Materials), Beijing, China.

Department of Pharmacognosy, Shanxi Medicine University, Taiyuan, China.

出版信息

FEBS Open Bio. 2022 Jul;12(7):1344-1352. doi: 10.1002/2211-5463.13412. Epub 2022 May 2.

DOI:10.1002/2211-5463.13412
PMID:35429231
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9249337/
Abstract

Chaihu, the dried roots of some species of Bupleurum L., is a famous Chinese herbal medicine for treatment of liver- and cold-related diseases, in which saikosaponins (SSs) are the major active compounds. Many of the genetic components upstream of SS biosynthetic pathways have been characterized; however, the regulatory mechanisms remain elusive. In this study we identified the APETALA2/Ethylene Responsive Factor family transcription factor gene BcERF3 from B. chinense. The expression of BcERF3 was induced in methyl-jasmonate-treated adventitious root of B. chinense; it was also expressed at higher levels in roots than in other tissues (stem, leaf, flower, and tender fruit of early fruiting plants). Transient expression of BcERF3 in the leaves of Nicotiana benthamiana resulted in intracellular localization of the protein in the nucleus. It was also demonstrated that the number of SSs was greater in BcERF3-overexpressing hairy roots of B. chinense than in plants treated with empty vector controls. This coincided with upregulation of β-AS, which encodes a key enzyme involved with triterpenoid biosynthesis. In conclusion, BcERF3 plays a positive regulatory role in the biosynthesis of SSs.

摘要

柴胡，一些柴胡属植物的干燥根，是一种著名的中草药，用于治疗肝脏和寒相关疾病，其中柴胡皂苷（SSs）是主要的活性化合物。许多 SS 生物合成途径的上游遗传成分已经得到了描述；然而，调控机制仍不清楚。在这项研究中，我们从 B. chinense 中鉴定出 APETALA2/乙烯响应因子家族转录因子基因 BcERF3。BcERF3 的表达在甲基茉莉酸处理的 B. chinense 不定根中被诱导；它在根中的表达水平也高于其他组织（茎、叶、花和早期结果植物的嫩果）。BcERF3 在烟草原生质体中的瞬时表达导致蛋白在核内的细胞内定位。还证明了 B. chinense 中 BcERF3 过表达毛状根中的 SSs 数量比用空载体对照处理的植物多。这与β-AS 的上调相一致，β-AS 编码参与三萜生物合成的关键酶。总之，BcERF3 对 SSs 的生物合成起着正向调控作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c33a/9249337/d8e00a6cbca9/FEB4-12-1344-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c33a/9249337/33f93b562cc3/FEB4-12-1344-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c33a/9249337/039175701b95/FEB4-12-1344-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c33a/9249337/ef5fe5457c57/FEB4-12-1344-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c33a/9249337/d8e00a6cbca9/FEB4-12-1344-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c33a/9249337/33f93b562cc3/FEB4-12-1344-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c33a/9249337/039175701b95/FEB4-12-1344-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c33a/9249337/ef5fe5457c57/FEB4-12-1344-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c33a/9249337/d8e00a6cbca9/FEB4-12-1344-g005.jpg

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