Department of Bioinformatics and Biochemistry, BRICS, Technische Universität Braunschweig, 38106 Braunschweig, Germany.
Department of Bioinformatics and Biochemistry, BRICS, Technische Universität Braunschweig, 38106 Braunschweig, Germany.
Curr Opin Biotechnol. 2022 Jun;75:102711. doi: 10.1016/j.copbio.2022.102711. Epub 2022 Apr 13.
Subcellular compartmentalization provides cells with tremendous advantages for the operation of cellular metabolism. Spatial separation of metabolism generates microenvironments with distinct concentrations of metabolites and cofactors allowing the cell to execute otherwise thermodynamically exclusive reactions simultaneously. Moreover, compartmentalization is also involved in the fine-tuned regulation of gene expression. Thus, the elucidation of compartment-specific metabolic processes has become essential for a thorough understanding of cellular metabolism. The limited availability of reliable methods to inspect metabolic activities in subcellular compartments has been a major impediment in this field. During the last decade, progress has been greatly extended through methodological advances in subcellular metabolic profiling. This review summarizes recent strategies applied for the direct and indirect assessment of compartment-specific metabolism. We review contemporary techniques, recent advances, limitations, and future trends in the field of subcellular metabolomics.
亚细胞区室化赋予了细胞在进行细胞代谢时巨大的优势。代谢的空间分离产生了具有不同代谢物和辅因子浓度的微环境，从而使细胞能够同时执行热力学上不相容的反应。此外，区室化还参与了基因表达的精细调控。因此，阐明特定于区室的代谢过程对于深入了解细胞代谢至关重要。在这个领域，缺乏可靠的方法来检测亚细胞区室中的代谢活性一直是一个主要障碍。在过去的十年中，通过亚细胞代谢组学方法的进展，这一进展得到了极大的扩展。本文总结了目前用于直接和间接评估区室特异性代谢的策略。我们回顾了亚细胞代谢组学领域的当代技术、最新进展、局限性和未来趋势。
