Key Laboratory of Jianghuai Agricultural Product Fine Processing and Resource Utilization Ministry of Agriculture and Rural Affairs, Anhui Engineering Laboratory for Agro-Products Processing, Food Processing Research Institute, College of Tea & Food Science and Technology, Anhui Agricultural University, Hefei 230036, China.
J Agric Food Chem. 2023 Nov 29;71(47):18510-18523. doi: 10.1021/acs.jafc.3c05713. Epub 2023 Nov 16.
In this study, galangin (Gal), kaempferol (Kae), quercetin (Que), and myricetin (Myr) were chosen as the representative flavonoids with different phenolic hydroxyl numbers in the B-ring. The edible dock protein (EDP) was chosen as the new plant protein. Based on this, the regulation mechanism of the phenolic hydroxyl number on the self-assembly behavior and molecular interaction between EDP and flavonoid components were investigated. Results indicated that the loading capacity order of flavonoids within the EDP nanomicelles was Myr (10.92%) > Que (9.56%) > Kae (6.63%) > Gal (5.55%). Moreover, this order was consistent with the order of the hydroxyl number in the flavonoid's B ring: Myr (3) > Que (2) > Kae (1) > Gal (0). The micro morphology exhibited that four flavonoid-EDP nanomicelles had a core-shell structure. In the meantime, the EDP encapsulation remarkably improved the flavonoids' water solubility, storage stability, and sustained release characteristics. During the interaction of EDP and flavonoids, the noncovalent interactions including van der Waals forces, hydrophobic interaction, and hydrogen bonding were the main binding forces. All of the results demonstrated that the hydroxyl number of bioactive compounds is a critical factor for developing a delivery system with high loading ability and stability.
在本研究中，选择姜黄素（Gal）、山奈酚（Kae）、槲皮素（Que）和杨梅素（Myr）作为具有不同 B 环酚羟基数的代表性类黄酮，选择食用码头蛋白（EDP）作为新的植物蛋白。在此基础上，研究了酚羟基数对 EDP 与类黄酮成分自组装行为和分子相互作用的调节机制。结果表明，类黄酮在 EDP 纳米胶束中的负载能力顺序为杨梅素（10.92%）>槲皮素（9.56%）>山奈酚（6.63%）>姜黄素（5.55%）。此外，该顺序与类黄酮 B 环中羟基数的顺序一致：杨梅素（3）>槲皮素（2）>山奈酚（1）>姜黄素（0）。微观形貌显示，四种黄酮-EDP 纳米胶束具有核壳结构。同时，EDP 包封显著提高了类黄酮的水溶性、储存稳定性和缓释特性。在 EDP 与类黄酮的相互作用中，范德华力、疏水相互作用和氢键等非共价相互作用是主要的结合力。所有结果表明，生物活性化合物的羟基数是开发具有高负载能力和稳定性的递送系统的关键因素。
