轴突上神经突的延伸受到针对特定神经细胞表面糖蛋白的抗体的损害。
Extension of neurites on axons is impaired by antibodies against specific neural cell surface glycoproteins.
作者信息
Chang S, Rathjen F G, Raper J A
出版信息
J Cell Biol. 1987 Feb;104(2):355-62. doi: 10.1083/jcb.104.2.355.
Abstract
We have developed an in vitro assay which measures the ability of growth cones to extend on an axonal substrate. Neurite lengths were compared in the presence or absence of monovalent antibodies against specific neural cell surface glycoproteins. Fab fragments of antibodies against the neural cell adhesion molecule, NCAM, have an insignificant effect on the lengths of neurites elongating on either an axonal substrate or a laminin substrate. Fab fragments of polyclonal antibodies against two new neural cell surface antigens, defined by mAb G4 and mAb F11, decrease the lengths of neurites elongating on an axonal substrate, but have no effect on the lengths of neurites elongating on a laminin substrate. G4 antigen is related to mouse L1, while F11 antigen appears to be distinct from all known neural cell surface glycoproteins. Our results suggest that the G4 and F11 antigens help to promote the extension of growth cones on axons.
摘要
我们开发了一种体外检测方法，用于测量生长锥在轴突底物上延伸的能力。在存在或不存在针对特定神经细胞表面糖蛋白的单价抗体的情况下，比较了神经突的长度。抗神经细胞粘附分子NCAM的抗体Fab片段，对在轴突底物或层粘连蛋白底物上伸长的神经突长度影响不显著。针对由单克隆抗体G4和单克隆抗体F11定义的两种新的神经细胞表面抗原的多克隆抗体Fab片段，会缩短在轴突底物上伸长的神经突长度，但对在层粘连蛋白底物上伸长的神经突长度没有影响。G4抗原与小鼠L1相关，而F11抗原似乎与所有已知的神经细胞表面糖蛋白不同。我们的结果表明，G4和F11抗原有助于促进生长锥在轴突上的延伸。
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