Johnson C D, Stretton A O
J Neurosci. 1985 Aug;5(8):1984-92. doi: 10.1523/JNEUROSCI.05-08-01984.1985.
Choline acetyltransferase (CAT) activity has been measured in the nematode Ascaris lumbricoides. Strips of hypodermal tissue which contained branches of single identified motoneurons (Stretton, A.O.W., R.M. Fishpool, E. Southgate, J.E. Donmoyer, J.P. Walrond, and I.S. Kass (1978) Proc. Natl. Acad. Sci. U.S.A. 75: 3493-3497) as well as control strips containing no neural tissue were assayed. In strips of hypodermis which contained branches of excitatory motoneurons (classes DE1, DE2, and DE3), CAT activity was elevated 5 to 10 times compared to control levels. Branches of inhibitory motoneurons (classes D1 and V1) did not add to the level of CAT observed in the controls. These data provide additional support for the suggestion (e.g., del Castillo, J., W.C. deMello, and T. Morales (1963) Experientia 20: 141) that acetylcholine is an excitatory neurotransmitter at nematode neuromuscular junctions. The function of the low level of CAT observed throughout the hypodermis is unknown.
已对线虫蛔虫的胆碱乙酰转移酶（CAT）活性进行了测定。对含有单个已识别运动神经元分支的皮下组织条带（斯特雷顿，A.O.W.，R.M.菲什普尔，E.索思盖特，J.E.唐莫耶，J.P.瓦尔隆德，以及I.S.卡斯（1978年）《美国国家科学院院刊》75: 3493 - 3497）以及不含神经组织的对照条带进行了检测。在含有兴奋性运动神经元（DE1、DE2和DE3类）分支的皮下组织条带中，CAT活性相较于对照水平升高了5至10倍。抑制性运动神经元（D1和V1类）的分支并未使对照中观察到的CAT水平增加。这些数据为如下观点（例如，德尔卡斯蒂略，J.，W.C.德梅洛，以及T.莫拉莱斯（1963年）《实验》20: 141）提供了更多支持，即乙酰胆碱是线虫神经肌肉接头处的兴奋性神经递质。在整个皮下组织中观察到的低水平CAT的功能尚不清楚。
