Walrond J P, Kass I S, Stretton A O, Donmoyer J E
J Neurosci. 1985 Jan;5(1):1-8. doi: 10.1523/JNEUROSCI.05-01-00001.1985.
A physiological preparation in which it is possible to record responses in muscle to stimulation of single motoneurons of the nematode Ascaris lumbricoides is described. With this preparation we have determined the physiological sign (E or I; excitatory or inhibitory) of the neuromuscular synapses of 21 identified motoneurons--12 are excitatory and 9 inhibitory. Ascaris motoneurons had previously been classified by morphological criteria into seven classes (Stretton, A. O. W., R. M. Fishpool, E. Southgate, J. E. Donmoyer, J. P. Walrond, J. E. R. Moses, and I. S. Kass (1978) Proc. Natl. Acad. Sci. U. S. A. 75: 3493-3497). Physiological studies were performed on members of five of these classes. Three classes of neurons (DE1, DE2, and DE3) are excitatory to dorsal muscle cells. Two classes (DI and VI) are inhibitory neurons which innervate the dorsal and ventral muscle cells, respectively. The motoneurons in Caenorhabditis elegans (White, J. E., E. Southgate, J. N. Thomson, and S. Brenner (1976) Philos. Trans. R. Soc. Lond. (Biol.) 275: 327-348) can be divided into seven morphological classes which are very similar to those in Ascaris. Based upon the structure-function correlation in Ascaris, we have predicted which motoneurons are excitatory and which are inhibitory in C. elegans.
本文描述了一种生理学制备方法，利用该方法可以记录线虫蛔虫单一运动神经元受到刺激时肌肉的反应。通过这种制备方法，我们确定了21个已识别运动神经元的神经肌肉突触的生理特征（E或I；兴奋性或抑制性），其中12个为兴奋性，9个为抑制性。蛔虫运动神经元此前已根据形态学标准分为七类（斯特雷顿，A.O.W.，R.M.菲什普尔，E.索思盖特，J.E.唐莫耶，J.P.沃尔隆德，J.E.R.摩西，以及I.S.卡斯（1978年）《美国国家科学院院刊》75:3493 - 3497）。对其中五类的成员进行了生理学研究。三类神经元（DE1、DE2和DE3）对背侧肌肉细胞具有兴奋性。两类（DI和VI）是抑制性神经元，分别支配背侧和腹侧肌肉细胞。秀丽隐杆线虫中的运动神经元（怀特，J.E.，E.索思盖特，J.N.汤姆森，以及S.布伦纳（1976年）《伦敦皇家学会哲学学报》（生物）275:327 - 348）可分为七个形态学类别，与蛔虫中的非常相似。基于蛔虫中的结构 - 功能相关性，我们预测了秀丽隐杆线虫中哪些运动神经元是兴奋性的，哪些是抑制性的。
