Mak Gannon Ck, Lau Stephen Sy, Wong Kitty Ky, Chow Nancy Ls, Lau Chi-Shan, Lam Edman Tk, Ng Ken Hl, Chan Rickjason Cw
Microbiology Division, Public Health Laboratory Services Branch, Centre for Health Protection, Department of Health, Hong Kong Special Administrative Region, China.
Future Virol. 2022 Mar. doi: 10.2217/fvl-2021-0229. Epub 2022 Apr 11.
Currently, there is lack of data regarding rapid antigen detection (RAD) kits to detect SARS-CoV-2 B.1.617.2 virus. The purpose of this evaluation is to assess analytical sensitivity of 12 RAD kits against SARS-CoV-2 B.1.617.2. Analytical sensitivity was determined by limit of detection (LOD). A serial tenfold dilution set from a respiratory specimen collected from a COVID-19 patient infected by SARS-CoV-2 B.1.617.2 was used. RT-PCR was used as a reference method. The LOD results showed that 11 and one RAD kits were 100- and 1000-fold less sensitive than RT-PCR respectively. The results showed that the RAD kits evaluated in this study may be used for first-line screening of the SARS-CoV-2 B.1.617.2 variant.
目前，缺乏关于检测严重急性呼吸综合征冠状病毒2（SARS-CoV-2）B.1.617.2变体的快速抗原检测（RAD）试剂盒的数据。本评估的目的是评估12种RAD试剂盒对SARS-CoV-2 B.1.617.2的分析灵敏度。分析灵敏度通过检测限（LOD）确定。使用从感染SARS-CoV-2 B.1.617.2的新冠肺炎患者采集的呼吸道标本进行连续十倍稀释。逆转录聚合酶链反应（RT-PCR）用作参考方法。检测限结果表明，11种和1种RAD试剂盒的灵敏度分别比RT-PCR低100倍和1000倍。结果表明，本研究中评估的RAD试剂盒可用于SARS-CoV-2 B.1.617.2变体的一线筛查。
