Tianle Chao, Yunhan Fan, Delong Lou, Haitao Xia, Lanting Ma, Xueqing Shan, Liuxu Yang, Yu He, Guizhi Wang
Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Shandong Agricultural University, Tai'an, Shandong, China.
Comprehensive Testing and Inspection Center, Shandong Provincial Animal Husbandry and Veterinary Bureau, Jinan, Shandong, China.
Anim Biotechnol. 2023 Dec;34(9):4175-4186. doi: 10.1080/10495398.2022.2061506. Epub 2022 Apr 18.
As a commonly used acaricide in apiculture, fluvalinate is used to kill Varroa mites, while it also damages the nervous system of honeybees. To date, the transcriptomic characteristics associated with fluvalinate-induced neuronal injury in the bee brain have not been reported. Here, we performed transcriptome sequencing on ligustica ( ligustica) brain tissues collected before and after fluvalinate treatment. A total of 546 differentially expressed genes (DEGs) were detected, and these DEGs mainly showed 4 different expression patterns. Further analysis revealed that DEGs with different expression patterns were mainly involved in lipid metabolism, amino acid metabolism, visual transduction, and neural response-related GO terms and KEGG pathways. Moreover, protein-protein interaction network analysis revealed five protein-coding DEGs as key genes, which may play important roles in the resistance to fluvalinate-induced honeybee brain nerve tissue damage. In summary, this study is the first to perform a detailed characterization and functional analysis of genes related to fluvalinate stimulation in honeybee brains.
作为养蜂业中常用的杀螨剂，氟胺氰菊酯用于杀死瓦螨，同时它也会损害蜜蜂的神经系统。迄今为止，尚未有关于氟胺氰菊酯诱导蜜蜂大脑神经元损伤相关的转录组特征的报道。在此，我们对经氟胺氰菊酯处理前后采集的意大利蜜蜂（Apis mellifera ligustica）脑组织进行了转录组测序。共检测到546个差异表达基因（DEGs），这些DEGs主要呈现4种不同的表达模式。进一步分析表明，具有不同表达模式的DEGs主要参与脂质代谢、氨基酸代谢、视觉转导以及与神经反应相关的基因本体（GO）术语和京都基因与基因组百科全书（KEGG）通路。此外，蛋白质-蛋白质相互作用网络分析揭示了5个蛋白质编码DEGs作为关键基因，它们可能在抵抗氟胺氰菊酯诱导的蜜蜂脑神经组织损伤中发挥重要作用。总之，本研究首次对蜜蜂大脑中与氟胺氰菊酯刺激相关的基因进行了详细的特征描述和功能分析。
