Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province and School of Chemistry and Chemical Engineering, Shaanxi Normal University, 620 Xi Chang'an Street, Xi'an, Shaanxi 710119, People's Republic of China.
Analyst. 2022 May 17;147(10):2060-2067. doi: 10.1039/d2an00294a.
Hypochlorite acid (ClO) is one of the major reactive oxygen species (ROS) in colon cancer, providing an effective target for colonic tumor imaging. For detection of ClO and tumor imaging, poly[(9,9-di(2-ethylhexyl)-9-fluorene-2,7-vinylene)--(1-methoxy-4-(2-ethylhexyloxy)-2,5-phenylenevinylene)] (PFV--MEHPV, namely CP1) was encapsulated in mesoporous silica nanoparticles (MSNs) that were pre-modified with polyphenylenevinylene (PPV) polymerization to construct bright PPV@MSN-CP1 nanoparticles. The synthesized nanoparticles were size-stable and not cytotoxic as confirmed by FE-TEM, FE-SEM, and MTT assay. Hypochlorite oxidizes the vinylidene bond of CP1 through π-π cycloaddition to form PPV-dioxetane intermediates to generate photons. The CL quantum yield of PPV@MSN-CP1 was 16.7 times higher than that of Pluronic F-127 wrapped CP1. CL nanoparticles PPV@MSN-CP1 have good selectivity for hypochlorite detection among biological oxidants (mainly ROS). The linear range and the LOD of PPV@MSN@CP1 for ClO detection are 4-90 and 1.02 μM, respectively. Subsequently, we further coated PPV@MSN@CP1 with folic acid for tumor targeting by phospholipid wrapping. PPV@MSN-CP1@FA was successfully applied for imaging of endogenously produced ClO of tumor tissue in living animals.
次氯酸（ClO）是结肠癌中主要的活性氧（ROS）之一，为结肠肿瘤成像提供了有效的靶点。为了检测 ClO 和肿瘤成像，将聚[（9,9-二（2-乙基己基）-9-芴-2,7-亚乙烯基）-（1-甲氧基-4-（2-乙基己氧基）-2,5-亚苯基亚乙烯基）]（PFV-MEHPV，即 CP1）封装在介孔硅纳米粒子（MSNs）中，这些 MSNs 预先用聚芴乙烯（PPV）聚合进行了修饰，以构建明亮的 PPV@MSN-CP1 纳米粒子。FE-TEM、FE-SEM 和 MTT 测定证实，所合成的纳米粒子尺寸稳定且无细胞毒性。次氯酸通过π-π环加成氧化 CP1 的亚乙烯基键，形成 PPV-二恶烷中间体，从而产生光子。PPV@MSN-CP1 的 CL 量子产率比 Pluronic F-127 包裹的 CP1 高 16.7 倍。CL 纳米粒子 PPV@MSN-CP1 对生物氧化剂（主要是 ROS）中的次氯酸盐检测具有良好的选择性。PPV@MSN@CP1 对 ClO 检测的线性范围和 LOD 分别为 4-90 和 1.02 μM。随后，我们进一步用磷脂包裹将 PPV@MSN@CP1 包覆上叶酸，用于肿瘤靶向。成功地将 PPV@MSN-CP1@FA 应用于活体动物肿瘤组织内源性产生的 ClO 的成像。
