Japan-Thailand Research Collaboration Center, Research Institute for Microbial Diseases, Osaka University, Suita, Japan.
Faculty of Public Health, Kasetsart University Chalermphrakiat Sakon Nakhon Province Campus, Sakon Nakhon, Thailand.
Microb Genom. 2022 Apr;8(4). doi: 10.1099/mgen.0.000797.
Carbapenem-resistant (CRE) are a serious public health threat because of their rapid dissemination. To determine the epidemiological and genetic characteristics of CRE infections in Thailand, we performed whole-genome sequencing of 577 carbapenem-resistant isolates and 170 carbapenem-resistant isolates from hospitals across the nation. The four most prevalent carbapenemase genes harboured by these bacteria were , , and . The gene was identified in diverse sequence types. The gene was identified almost exclusively in . The genes , , and co-carriage of and were found in specific sequence types from certain provinces. Replicon typing revealed the diverse backbones of - and -harbouring plasmids and successful expansion of -harbouring IncN2-type plasmids. Core-genome single-nucleotide polymorphism analysis suggested that -, -, -, and co-carriage of and -associated sub-clonal lineages have recently predominated in the provinces from where these isolates were isolated. Thus, we demonstrate genotype-dependent dissemination of CRE in Thailand, which is helpful for establishing infection-control strategies in CRE-endemic areas.
耐碳青霉烯肠杆菌（CRE）由于其快速传播，对公共健康构成严重威胁。为了确定泰国耐碳青霉烯肠杆菌感染的流行病学和遗传特征，我们对来自全国各地医院的 577 株耐碳青霉烯肠杆菌和 170 株耐碳青霉烯肠杆菌进行了全基因组测序。这些细菌携带的四种最常见的碳青霉烯酶基因是 、 、 和 。基因 存在于多种序列类型中。基因 几乎只存在于 中。基因 、 和 与 或 共同携带存在于某些省份的特定序列类型中。复制子分型显示了携带 和 的质粒的不同骨架，并成功扩展了携带 IncN2 型质粒的 。核心基因组单核苷酸多态性分析表明， -、 -、 -和 与 相关的亚克隆谱系最近在这些分离株分离的省份中占主导地位。因此，我们证明了泰国耐碳青霉烯肠杆菌的基因型依赖性传播，这有助于在耐碳青霉烯肠杆菌流行地区建立感染控制策略。
