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携带IncN2质粒的临床序列型405的基因型和表型特征

Genotypic and Phenotypic Characterization of Clinical Sequence Type 405 Carrying IncN2 Plasmid Harboring .

作者信息

Hao Yingying, Shao Chunhong, Geng Xu, Bai Yuanyuan, Jin Yan, Lu Zhiming

机构信息

Department of Clinical Laboratory, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, China.

出版信息

Front Microbiol. 2019 Apr 12;10:788. doi: 10.3389/fmicb.2019.00788. eCollection 2019.

DOI:10.3389/fmicb.2019.00788

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6499153/

Abstract

We report a -carrying ST405 recovered from the abdominal fluid of a patient in Shandong, China. This strain belonged to the high-risk phylogenetic group D and carried the virulence genes, , and iroN. In addition to , this isolate carried the quinolone resistance gene and extended-spectrum β-lactamase (ESBL) genes and . was located on a 41 Kb IncN2 self-transmissible plasmid. The IncN2 plasmid named as pJN24NDM1 was fully sequenced and analyzed. Genome comparative analysis showed that IncN2 plasmids harboring carbapenem-resistance genes possessed conserved backbones and variable accessory regions. Phylogenetic analysis of 87 IncN plasmids based on orthologous genes indicated that 9 IncN2 plasmids fell into one phylogenetic clade, while 4 IncN3 plasmids were in two phylogenetic clades of the 74 IncN1 plasmids. The presence of IncN2 plasmids harboring in the high-risk clone ST405 raises serious concerns for its potential of dissemination.

摘要

我们报告了从中国山东一名患者腹腔积液中分离出的携带NDM-1的ST405菌株。该菌株属于高风险系统发育组D，携带毒力基因hlyA、fyuA和iroN。除了NDM-1，该分离株还携带喹诺酮耐药基因qnrS和超广谱β-内酰胺酶（ESBL）基因blaCTX-M-1和blaTEM-1。NDM-1位于一个41 Kb的IncN2自我传递质粒上。对名为pJN24NDM1的IncN2质粒进行了全序列测定和分析。基因组比较分析表明，携带碳青霉烯耐药基因的IncN2质粒具有保守的主干和可变的辅助区域。基于直系同源基因对87个IncN质粒进行的系统发育分析表明，9个IncN2质粒属于一个系统发育分支，而4个IncN3质粒则分布在74个IncN1质粒的两个系统发育分支中。在高风险克隆ST405菌株中存在携带NDM-1的IncN2质粒，这对其传播潜力引发了严重担忧。

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