Brown J, Greenwood B M, Terry R J
Parasite Immunol. 1986 Nov;8(6):551-64. doi: 10.1111/j.1365-3024.1986.tb00869.x.
This paper reports the results of in vitro experiments which attempt to elucidate the mechanisms whereby Gambian children control acute infections of Plasmodium falciparum. It was shown initially that mononuclear cells from children with acute malaria, in the presence of specific antibody, caused a marked reduction in in vitro parasite growth. IgM antibodies appeared to be considerably more effective than IgG. T or B lymphocytes were ineffective in the system; adherent cells alone had some effect, but much less than the unfractionated cell population. Adherent cells were however fully effective after exposure to supernatants from T cells activated either non-specifically by phytohaemagglutinin (PHA), or specifically by P. falciparum antigens. Depression of parasite growth was also observed, independent of anti-malarial antibody. This was achieved when adherent cells from healthy Europeans, as well as those from infected children, were exposed to the supernatants from previously stimulated T cells before adding to the culture. Furthermore, intra-erythrocytic parasite death occurred after a short exposure to the supernatants of 'activated' adherent cells from both infected children and Europeans.
本文报告了体外实验的结果，这些实验试图阐明冈比亚儿童控制恶性疟原虫急性感染的机制。最初发现，在特异性抗体存在的情况下，急性疟疾患儿的单核细胞可使体外寄生虫生长显著减少。IgM抗体似乎比IgG抗体有效得多。在该系统中，T淋巴细胞或B淋巴细胞无效；单独的贴壁细胞有一定作用，但远不如未分离的细胞群体。然而，贴壁细胞在暴露于由植物血凝素（PHA）非特异性激活或由恶性疟原虫抗原特异性激活的T细胞的上清液后，效果完全显现。在不依赖抗疟抗体的情况下，也观察到了寄生虫生长的抑制。当来自健康欧洲人的贴壁细胞以及来自感染儿童的贴壁细胞在加入培养物之前暴露于先前刺激的T细胞的上清液时，即可实现这一点。此外，在短时间暴露于来自感染儿童和欧洲人的“活化”贴壁细胞的上清液后，红细胞内的寄生虫死亡。
