Chembio Diagnostic Systems, Inc., Medford, New York 11763, USA.
Centro de Vigilancia Sanitaria Veterinaria (VISAVET) and Animal Health Department, Veterinary Faculty, Universidad Complutense de Madrid, Madrid, Spain.
Vet Immunol Immunopathol. 2022 Jun;248:110419. doi: 10.1016/j.vetimm.2022.110419. Epub 2022 Apr 8.
Bovine tuberculosis (bTB) control programs can be improved by combined use of tests for humoral and cell-mediated immune responses targeting multiple biomarkers of Mycobacterium bovis. To further the diagnostic benefits of this approach, we used Dual Path Platform (DPP) technology to test sera from cattle with naturally acquired bTB in the United States (US) and Spain for the presence of M. bovis antigen, IgM and/or IgG antibodies to MPB70/MPB83 fusion antigen in conjunction with tuberculin skin tests (TST) or interferon-gamma release assays (IGRA). When TST was complemented with detection of IgM and IgG antibodies, the diagnostic sensitivity increased from 85.4% to 95.1% in the US and from 64.2% to 81.5% in Spain. Likewise, adding the DPP assays enhanced IGRA diagnostic sensitivity from 82.7% to 93.8% in Spain. Detection of circulating M. bovis antigen showed added value when used in combination with the DPP antibody assays but it was limited when analyzed in the context of TST or IGRA results. Present findings support the benefits of a multi-test approach for the ante-mortem diagnosis of bTB in cattle.
牛结核病（bTB）控制计划可以通过联合使用针对多种牛分枝杆菌生物标志物的体液和细胞介导免疫反应测试来改善。为了进一步提高这种方法的诊断效果，我们使用双重路径平台（DPP）技术，对来自美国和西班牙的自然感染 bTB 的牛血清进行检测，以检测 M. bovis 抗原、MPB70/MPB83 融合抗原的 IgM 和/或 IgG 抗体，同时结合结核菌素皮肤试验（TST）或干扰素-γ释放试验（IGRA）。当 TST 与 IgM 和 IgG 抗体检测相结合时，美国的诊断敏感性从 85.4%增加到 95.1%，西班牙的诊断敏感性从 64.2%增加到 81.5%。同样，在西班牙，添加 DPP 检测可将 IGRA 的诊断敏感性从 82.7%提高到 93.8%。当与 DPP 抗体检测联合使用时，检测循环 M. bovis 抗原显示出了额外的价值，但在分析 TST 或 IGRA 结果时，其价值有限。目前的研究结果支持在牛的生前诊断中采用多测试方法来检测 bTB 的优势。
