快速抗体检测用于识别结核菌素皮肤试验结果无反应的患结核病牛的潜力。
Potential for rapid antibody detection to identify tuberculous cattle with non-reactive tuberculin skin test results.
作者信息
Waters W Ray, Vordermeier H Martin, Rhodes Shelley, Khatri Bhagwati, Palmer Mitchell V, Maggioli Mayara F, Thacker Tyler C, Nelson Jeffrey T, Thomsen Bruce V, Robbe-Austerman Suelee, Bravo Garcia Doris M, Schoenbaum Mark A, Camacho Mark S, Ray Jean S, Esfandiari Javan, Lambotte Paul, Greenwald Rena, Grandison Adrian, Sikar-Gang Alina, Lyashchenko Konstantin P
机构信息
National Animal Disease Center, Agricultural Research Service, United States Department of Agriculture (USDA), Ames, IA, USA.
Tuberculosis Research Group, Animal and Plant Health Agency, Addlestone, UK.
出版信息
BMC Vet Res. 2017 Jun 7;13(1):164. doi: 10.1186/s12917-017-1085-5.
BACKGROUND
Bovine tuberculosis (TB) control programs generally rely on the tuberculin skin test (TST) for ante-mortem detection of Mycobacterium bovis-infected cattle.
RESULTS
Present findings demonstrate that a rapid antibody test based on Dual-Path Platform (DPP) technology, when applied 1-3 weeks after TST, detected 9 of 11 and 34 of 52 TST non-reactive yet M. bovis-infected cattle from the US and GB, respectively. The specificity of the assay ranged from 98.9% (n = 92, US) to 96.0% (n = 50, GB) with samples from TB-free herds. Multi-antigen print immunoassay (MAPIA) revealed the presence of antibodies to multiple antigens of M. bovis in sera from TST non-reactors diagnosed with TB.
CONCLUSIONS
Thus, use of serologic assays in series with TST can identify a significant number of TST non-reactive tuberculous cattle for more efficient removal from TB-affected herds.
背景
牛结核病（TB）控制计划通常依靠结核菌素皮肤试验（TST）对感染牛分枝杆菌的牛进行宰前检测。
结果
目前的研究结果表明，一种基于双路径平台（DPP）技术的快速抗体检测方法，在TST后1至3周应用时，分别从美国和英国检测出11头TST无反应但感染牛分枝杆菌的牛中的9头，以及52头中的34头。该检测方法对来自无结核病牛群的样本的特异性范围为98.9%（n = 92，美国）至96.0%（n = 50，英国）。多抗原印迹免疫分析（MAPIA）显示，在被诊断为结核病的TST无反应者的血清中存在针对牛分枝杆菌多种抗原的抗体。
结论
因此，将血清学检测与TST串联使用，可以识别出大量TST无反应的结核病牛，以便更有效地从受结核病影响的牛群中清除。
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