Sridhara Archana A, Johnathan-Lee Ashley, Elahi Rubyat, Lambotte Paul, Esfandiari Javan, Boschiroli Maria Laura, Kerr Tanya J, Miller Michele A, Holder Thomas, Jones Gareth, Vordermeier H Martin, Marpe Breanne N, Thacker Tyler C, Palmer Mitchell V, Waters W Ray, Lyashchenko Konstantin P
Chembio Diagnostic Systems, Inc., Medford, NY 11763, USA.
Laboratory for Animal Health, Tuberculosis National Reference Laboratory, University Paris-Est, ANSES, Maisons-Alfort, France.
Vet Immunol Immunopathol. 2022 Nov;253:110499. doi: 10.1016/j.vetimm.2022.110499. Epub 2022 Oct 4.
Recent studies have suggested the potential of innovative serologic tests for accurate and rapid detection of bovine tuberculosis (bTB). Dual Path Platform (DPP) technology has been used to develop rapid animal-side antibody tests for Mycobacterium bovis infection in a range of livestock and wildlife host species. The present study evaluated diagnostic performance of DPP BovidTB IgM/IgG assay designed for differential detection of bovine IgM and IgG antibodies against two chimeric antigens, DID38 and TBf2, respectively, using 662 well-characterized serum samples from M. bovis-infected and bTB-free cattle collected in the United States, Great Britain, France, and South Africa. Test sensitivity and specificity ranged from 71% to 100% and from 95% to 100%, respectively, depending on the country, with overall accuracy of 83%. No significant risk of cross-reactivity with serum samples from cattle infected with most relevant species of mycobacteria other than M. bovis was found. The DPP BovidTB IgM/IgG assay may be suitable for use in multi-test algorithms to improve current strategies for bTB surveillance.
近期研究表明，创新的血清学检测方法在准确、快速检测牛结核病（bTB）方面具有潜力。双路径平台（DPP）技术已被用于开发针对一系列家畜和野生动物宿主物种中牛分枝杆菌感染的快速现场动物抗体检测方法。本研究使用从美国、英国、法国和南非收集的662份特征明确的牛分枝杆菌感染牛和无bTB牛的血清样本，评估了DPP牛科动物结核病IgM/IgG检测法的诊断性能，该检测法旨在分别针对两种嵌合抗原DID38和TBf2，鉴别检测牛的IgM和IgG抗体。根据国家不同，检测灵敏度和特异性分别为71%至100%和95%至100%，总体准确率为83%。未发现与感染除牛分枝杆菌外最相关分枝杆菌物种的牛血清样本发生交叉反应的显著风险。DPP牛科动物结核病IgM/IgG检测法可能适用于多检测算法，以改进当前的bTB监测策略。
