Department of Biomedical Engineering, Islamic Azad University, Central Tehran Branch, Tehran, Iran.
Department of Polymer Engineering & Color Technology, Amirkabir University of Technology, Tehran, Iran.
J Biomed Mater Res B Appl Biomater. 2022 Oct;110(10):2217-2230. doi: 10.1002/jbm.b.35071. Epub 2022 Apr 20.
Nanocomposites containing clay nanoparticles often present favorable properties such as good mechanical and thermal properties. They frequently have been studied for tissue engineering (TE) and regenerative medicine applications. On the other hand, poly(glycerol sebacate) (PGS), a revolutionary bioelastomer, has exhibited substantial potential as a promising candidate for biomedical application. Here, we present a facile approach to synthesizing stiff, elastomeric nanocomposites from sodium-montmorillonite nano-clay (MMT) in the commercial name of Cloisite Na and poly(glycerol sebacate urethane) (PGSU). The strong physical interaction between the intercalated Cloisite Na platelets and PGSU chains resulted in desirable property combinations for TE application to follow. The addition of 5% MMT nano-clay resulted in an over two-fold increase in the tensile modulus, increased the onset thermal decomposition temperature of PGSU matrix by 18°C, and noticeably improved storage modulus of the prepared scaffolds, compared with pure PGSU. As well, Cloisite Na enhanced the hydrophilicity and water uptake ability of the samples and accelerated the in-vitro biodegradation rate. Finally, in-vitro cell viability assay using L929 mouse fibroblast cells indicated that incorporating Cloisite Na nanoparticles into the PGSU network could improve the cell attachment and proliferation, rendering the synthesized bioelastomers potentially suitable for TE and regenerative medicine applications.
含有粘土纳米颗粒的纳米复合材料通常具有良好的机械和热性能等有利特性。它们经常被用于组织工程（TE）和再生医学应用的研究。另一方面，聚（甘油癸二酸酯）（PGS）是一种革命性的生物弹性体，作为生物医学应用的有前途的候选材料，已显示出巨大的潜力。在这里，我们提出了一种简便的方法，即用商业名称为 Cloisite Na 的钠蒙脱石纳米粘土（MMT）和聚（甘油癸二酸酯尿烷）（PGSU）合成刚性弹性纳米复合材料。插层 Cloisite Na 薄片和 PGSU 链之间的强物理相互作用导致了 TE 应用所需的理想性能组合。与纯 PGSU 相比，添加 5%的 MMT 纳米粘土可使拉伸模量增加一倍以上，使 PGSU 基体的起始热分解温度升高 18°C，并明显提高了所制备支架的储能模量。此外，Cloisite Na 提高了样品的亲水性和吸水性，并加速了体外生物降解速率。最后，使用 L929 小鼠成纤维细胞进行的体外细胞活力测定表明，将 Cloisite Na 纳米颗粒掺入 PGSU 网络中可以提高细胞的附着和增殖能力，使合成的生物弹性体有可能适用于 TE 和再生医学应用。
