Department of Molecular Biology, Pasteur Institute of Iran, Tehran, Iran.
Department of Molecular Biology, Central Veterinary Laboratory, Iranian Veterinary Organization, Tehran, Iran.
Arch Microbiol. 2022 Apr 20;204(5):269. doi: 10.1007/s00203-022-02840-x.
Salmonella is one of the most common causes of foodborne outbreaks and infection worldwide. The gold-standard detection method of Salmonella is cultivation. There is a need to investigate rapid and accurate processes with time-consuming cultivation. The study evaluated different approaches to detect Salmonella in poultry feces samples. Poultry farm feces samples from 21 cities in Iran were collected from January 2016 to December 2019. Microbiological cultures, serological assays, and multiplex PCR (m-PCR) were used to detect and characterize Salmonella spp. isolates. Serological assays and m-PCR were used to determine the serogroups A, B, C1, C2, D1, E, H, and FliC. The m-PCR was used to detect seven Salmonella serovars, and a Chi-square test was performed to compare the discriminatory power of the methods. Of 2300 poultry feces samples, 173 (7.5%) and 166 (7.2%) samples were detected as Salmonella spp. by cultivation and m-PCR, respectively. The sensitivity of the molecular method was equal to cultivation at 0.96 (CI = 95%). Assessment of H antigenic subgroups showed the same for both m-PCR and serological tests. Therefore, the matching rate of the two methods for detecting all H antigenic subgroups was 100%. Thus, the relationship between the results obtained from both methods was significant in the contingency table test (P < 0.01). The PCR-based approach confirmed the detection of Salmonella in a shorter period (24-36 h) compared to the conventional microbiological approach (3-8 days).
沙门氏菌是世界范围内最常见的食源性疾病爆发和感染原因之一。沙门氏菌的金标准检测方法是培养。需要研究快速、准确的方法来替代耗时的培养。本研究评估了不同方法检测禽类粪便样本中沙门氏菌的效果。从 2016 年 1 月至 2019 年 12 月，从伊朗 21 个城市的家禽养殖场采集粪便样本。采用微生物培养、血清学检测和多重 PCR（m-PCR）来检测和鉴定沙门氏菌属分离株。采用血清学检测和 m-PCR 来确定血清型 A、B、C1、C2、D1、E、H 和 FliC。m-PCR 用于检测七种沙门氏菌血清型，并进行卡方检验比较方法的鉴别能力。在 2300 份家禽粪便样本中，培养法和 m-PCR 分别检出 173（7.5%）和 166（7.2%）份沙门氏菌属样本。分子方法的灵敏度与培养法相当，为 0.96（95%置信区间）。H 抗原亚型评估表明，m-PCR 和血清学检测结果相同。因此，两种方法检测所有 H 抗原亚型的符合率为 100%。因此，两种方法在列联表检验中的结果关系具有统计学意义（P<0.01）。与传统的微生物方法（3-8 天）相比，基于 PCR 的方法可在更短的时间（24-36 小时）内确认沙门氏菌的检测。
