Engel Yael, Silva Camila Vieira da, Ramos-Oliveira Thayanne Monteiro, Mantilla Taís Fonseca, Tavares Juliane de Paula, Freitas Patricia Moreira de
Universidade de São Paulo - USP, School of Dentistry, Department of Restorative Dentistry, São Paulo, SP, Brazil.
Universidade Tiradentes, School of Dentistry, Department of Restorative Dentistry, Aracaju, SE, Brazil.
Braz Oral Res. 2022 Apr 15;36:e054. doi: 10.1590/1807-3107bor-2022.vol36.0054. eCollection 2022.
This in vitro study evaluated the potential of CO2 laser (10.6 μm) combined with a stannous/fluoride-containing solution for preventing erosion in human/bovine enamel. Forty-eight samples of each substrate were randomly allocated to four groups (n = 12): W - distilled water; E - AmF/NaF/SnCl2 solution; L - CO2 laser; and LE - CO2 laser+AmF/NaF/SnCl2 solution. After surface treatments, samples were submitted to a 5-day erosive challenge, alternating immersions in 0.5% citric acid (2 minutes, 6x/day) and in artificial saliva. Optical profilometry (μm) and scanning electron microscopy (SEM) were used to determine surface loss and surface morphology, respectively. Data were statistically analyzed by two-way ANOVA and Tukey's tests (p < 0.05). For human enamel, tissue loss was lower in group L (12.37 ± 4.46) than in group W (16.45 ± 2.76), and higher than in the groups treated with AmF/NaF/SnCl2 solution (E-5.44 ± 2.37; LE-5.55 ± 2.31). In group L, SEM images reveled a disorganized surface but fewer projections than in group W and LE showed fewer irregularities than W, E, and L. For bovine enamel, tissue loss in group L (13.90 ± 3.50) did not differ from that in group W (14.10 ± 2.98), and was higher than losses in groups E (5.70 ± 2.12) and LE (8.12 ± 2.56), which were statistically similar to each other. Groups W and L had similar aspects of demineralization, whereas groups E and LE showed homogenous surfaces. Surface-treated samples had no changes in their surfaces. CO2 laser was able to slightly prevent surface loss only on human enamel surface, but did not enhance the AmF/NaF/SnCl2 effect on the prevention of enamel erosion.
这项体外研究评估了二氧化碳激光（10.6μm）联合含亚锡/氟溶液预防人/牛牙釉质侵蚀的潜力。每种基质的48个样本被随机分为四组（n = 12）：W - 蒸馏水；E - 胺氟/氟化钠/氯化亚锡溶液；L - 二氧化碳激光；LE - 二氧化碳激光 + 胺氟/氟化钠/氯化亚锡溶液。表面处理后，样本接受为期5天的侵蚀性挑战，交替浸泡在0.5%柠檬酸（2分钟，每天6次）和人工唾液中。分别使用光学轮廓仪（μm）和扫描电子显微镜（SEM）来测定表面损失和表面形态。数据通过双向方差分析和Tukey检验进行统计学分析（p < 0.05）。对于人牙釉质，L组（12.37 ± 4.46）的组织损失低于W组（16.45 ± 2.76），但高于用胺氟/氟化钠/氯化亚锡溶液处理的组（E - 5.44 ± 2.37；LE - 5.55 ± 2.31）。在L组中，SEM图像显示表面无序，但突起比W组少，LE组显示的不规则性比W、E和L组少。对于牛牙釉质，L组（13.90 ± 3.50）的组织损失与W组（14.10 ± 2.98）无差异，且高于E组（5.70 ± 2.12）和LE组（8.12 ± 2.56），后两组在统计学上彼此相似。W组和L组的脱矿情况相似，而E组和LE组显示表面均匀。经表面处理的样本表面无变化。二氧化碳激光仅能略微防止人牙釉质表面的损失，但不能增强胺氟/氟化钠/氯化亚锡溶液对预防牙釉质侵蚀的效果。
