Norris S J, Edmondson D G
Sex Transm Dis. 1986 Oct-Dec;13(4):207-13. doi: 10.1097/00007435-198610000-00001.
The nature of the serum requirement of Treponema pallidum subspecies pallidum (Nichols strain) was examined in a culture system utilizing Sf1Ep cottontail rabbit cells. In this system, significant multiplication of treponemes occurs in the presence of select lots of fetal bovine serum (FBS) or calf serum (CS) at concentrations of greater than or equal to 5% (vol/vol). Heat-inactivation of the serum greatly enhances treponemal multiplication, and normal human serum was found to be as effective as FBS in supporting the growth of T. pallidum. The protein fraction of FBS obtained by membrane ultrafiltration was capable of supporting the multiplication of T. pallidum when added to the basal tissue culture medium; an average increase of 23-fold was observed in these cultures, as compared with a mean increase of 25-fold in the 20% FBS controls. In contrast, the ultrafiltrate fraction of FBS (consisting of compounds with molecular weights of less than 10,000 daltons) did not support either growth or the retention of motility. Proteins precipitable with 25% (wt/vol) polyethylene glycol (i.e., albumin, transferrin, ceruloplasmin, and other proteins) also promoted the growth of T. pallidum. This observation provides further evidence that the required serum components are associated with the protein fraction.
利用Sf1Ep棉尾兔细胞培养系统，研究了梅毒螺旋体苍白亚种（Nichols株）对血清需求的性质。在该系统中，当存在特定批次的胎牛血清（FBS）或小牛血清（CS）且浓度大于或等于5%（体积/体积）时，梅毒螺旋体大量繁殖。血清热灭活极大地增强了梅毒螺旋体的繁殖，并且发现正常人血清在支持梅毒螺旋体生长方面与FBS一样有效。通过膜超滤获得的FBS蛋白组分添加到基础组织培养基中时能够支持梅毒螺旋体的繁殖；与20%FBS对照组平均增加25倍相比，这些培养物中观察到平均增加23倍。相比之下，FBS的超滤组分（由分子量小于10,000道尔顿的化合物组成）既不支持生长也不支持运动性的维持。用25%（重量/体积）聚乙二醇可沉淀的蛋白质（即白蛋白、转铁蛋白、铜蓝蛋白和其他蛋白质）也促进了梅毒螺旋体的生长。这一观察结果进一步证明所需的血清成分与蛋白质组分有关。
