通过脾内途径用囊胚对小鼠进行免疫的实验。

Experiments with immunization of mice with blastocysts by an intrasplenic route.

作者信息

Nilsson B O, Grönvik K O, Svalander P C

出版信息

Ups J Med Sci. 1983;88(2):151-3. doi: 10.3109/03009738309178448.

Abstract

A few attaching blastocysts from CBA/H mice were irradiated from a Cesium source and transferred into the spleen of male DBA2 mice. A booster immunization was performed after four weeks. Blood samples for preparation of antiserum to test for the presence of immunoglobulins directed against blastocyst surface determinants were obtained by a retro-orbital puncture. Specific antibodies were detected with a protein A-gold method, modified for transmission electron microscopy of air-dried blastocysts. The results showed that CBA/H blastocyst incubated in DBA2 immune serum were positive for protein A-gold labelling, while control blastocysts only possessed a few irregularly scattered gold particles. Thus, it seems as a deposition of antigens in the spleen tissue with persistence of the antigens at this site will result in detectable antibodies in the peripheral blood.

摘要

将来自CBA/H小鼠的一些着床囊胚用铯源照射后，移植到雄性DBA2小鼠的脾脏中。四周后进行加强免疫。通过眶后穿刺采集血样以制备抗血清，用于检测针对囊胚表面决定簇的免疫球蛋白的存在。采用蛋白质A-金法检测特异性抗体，该方法针对风干囊胚的透射电子显微镜观察进行了改良。结果显示，在DBA2免疫血清中孵育的CBA/H囊胚蛋白质A-金标记呈阳性，而对照囊胚仅含有一些不规则散在的金颗粒。因此，似乎抗原在脾脏组织中的沉积以及该部位抗原的持续存在会导致外周血中出现可检测到的抗体。