A and harboring strain reported and genomic characteristics of .

As a newly emerging pathogen, more and more drug resistant strains have been reported in recent years, which posed serious threats to public health. Here we first reported a multidrug-resistant strain 12084 with two and one genes isolated from the sputum specimen of a patient in the Second Affiliated Hospital of Zhejiang University School of Medicine and analyzed its genetic basis and drug-resistance phenotypes. Genetic analysis showed that this strain harbored three different incompatibility groups (IncHI2, IncHI5, and IncFII:IncFIB-4.1) of plasmids (p12084-HI2, p12084-HI5, and p12084-FII). A total of 26 drug-resistance genes belonging to 12 classes of antibiotics were identified, most of which (24) were located on two plasmids (p12084-HI2 and p12084-HI5). Interestingly, two genes were identified to locate on p12084-HI2 and p12084-HI5, respectively, both of which were embedded in In630, indicating their genetic homogeny. It was noting that one gene was situated in a novel unit transposon (referred to as Tn) on the p12084-HI5 plasmid. We also discovered an gene on the p12084-HI2 plasmid. To the best of our knowledge, this is the first report of a and harboring strain. We then investigated the population structure/classification, and antibiotic resistance for all 275 availably global genomes. Population structure revealed that could be divided into two main clades (Clade 1 and Clade 2); the most popular ST29 was located in Clade 1, while other common STs (such as ST50, ST27, and ST43) were located in Clade 2. Drug-resistance analysis showed 25.5% of the strains (70/275) harboring at least one carbapenemase gene, indicating severe drug resistance of beyond our imagination; this is a dangerous trend and should be closely monitored, especially for ST27 with the most drug-resistant genes among all the STs. Overall, we reported a and harboring strain, and further revealed the population structure/classification, and drug-resistance of , which provided an important framework, reference, and improved understanding of .