Association of Single-Nucleotide Polymorphisms of Gene with Nonsyndromic Cleft Lip with or without Cleft Palate in Kinh Vietnamese Patients.

Cleft lip with or without palate (CL/P) is the most common orofacial birth defect. Single-nucleotide polymorphisms (SNPs) in gene (V-Maf avian musculoaponeurotic fibrosarcoma oncogene homolog B) were identified as susceptible to this defect in a genome-wide association study. To further evaluate its role in this birth defect, we conducted this study with the aim of identifying allele frequencies, genotype frequencies, and association of SNPs rs13041247, rs6065259, and rs6072081 of gene with nonsyndromic cleft lip/palate (NCL/P) in Kinh Vietnamese patients.  We performed case-control study involved 79 patients with NCL/P and 77 healthy controls. DNAs were extracted from participants' saliva and tetra-amplification refractory mutation system polymerase chain reaction (tetra-ARMS PCR) was used for genotyping SNPs.  SNPs of gene were genotyped using the Tetra-ARMS PCR method. We found that genotype CT of rs13041247 was associated with an increased risk of NCL/P in Kinh Vietnamese (odds ratio [OR ] = 1.63, 95% confidence interval [CI] = 0.83-3.19,  = 0.17). The G allele genotypes of SNP rs6072081 increase high risk for the malformation, statistically significant result (OR  = 7.06, 95% CI = 2.13-23.42,  < 0.001). There is no clear association between rs6065259 and CL/P (OR  = 0.75, 95% CI = 0.22-2.50,  = 0.32; OR  = 1.53, 95% CI = 0.79-2.97,  = 0.32). When the patients were divided into the phenotypic subgroups, there was a similar significant trend between the patients and controls for all SNPs.  Our study provides further evidence of role of gene variations with NCL/P defect in Kinh Vietnamese.