Liu Yancheng, Takagi Yuko, Sugijanto Milyadi, Nguyen Kieu Duong My, Hirata Akira, Hori Hiroyuki, Ho C Kiong
Human Biology Program, University of Tsukuba, Tsukuba, Japan.
Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology, Tsukuba, Japan.
Front Mol Biosci. 2022 Mar 25;9:811548. doi: 10.3389/fmolb.2022.811548. eCollection 2022.
RNA ligases play important roles in repairing and circularizing RNAs post-transcriptionally. In this study, we generated an allelic knockout of ATP-dependent RNA ligase (Rnl) in the hyperthermophilic archaeon to identify its biological targets. A comparative analysis of circular RNA reveals that the Rnl-knockout strain represses circularization of C/D box sRNAs without affecting the circularization of tRNA and rRNA processing intermediates. Recombinant archaeal Rnl could circularize C/D box sRNAs with a mutation in the conserved C/D box sequence element but not when the terminal stem structures were disrupted, suggesting that proximity of the two ends could be critical for intramolecular ligation. Furthermore, accumulates aberrant RNA fragments derived from ribosomal RNA in the absence of Rnl. These results suggest that Rnl is responsible for C/D box sRNA circularization and may also play a role in ribosomal RNA processing.
RNA连接酶在转录后修复和环化RNA过程中发挥着重要作用。在本研究中，我们在嗜热古菌中产生了ATP依赖性RNA连接酶（Rnl）的等位基因敲除，以确定其生物学靶点。对环状RNA的比较分析表明，Rnl基因敲除菌株抑制了C/D盒小RNA的环化，而不影响tRNA和rRNA加工中间体的环化。重组古菌Rnl可以使保守C/D盒序列元件发生突变的C/D盒小RNA环化，但当末端茎结构被破坏时则不能，这表明两端的接近度对于分子内连接可能至关重要。此外，在没有Rnl的情况下，会积累来自核糖体RNA的异常RNA片段。这些结果表明，Rnl负责C/D盒小RNA的环化，并且可能在核糖体RNA加工中也发挥作用。
