Breuer Ruth, Gomes-Filho Jose-Vicente, Randau Lennart
Prokaryotic RNA Biology, Philipps-Universität Marburg, Marburg, Germany.
Front Microbiol. 2021 Mar 12;12:654029. doi: 10.3389/fmicb.2021.654029. eCollection 2021.
Post-transcriptional modifications fulfill many important roles during ribosomal RNA maturation in all three domains of life. Ribose 2'--methylations constitute the most abundant chemical rRNA modification and are, for example, involved in RNA folding and stabilization. In archaea, these modification sites are determined by variable sets of C/D box sRNAs that guide the activity of the rRNA 2'--methyltransferase fibrillarin. Each C/D box sRNA contains two guide sequences that can act in coordination to bridge rRNA sequences. Here, we will review the landscape of archaeal C/D box sRNA genes and their target sites. One focus is placed on the apparent accelerated evolution of guide sequences and the varied pairing of the two individual guides, which results in different rRNA modification patterns and RNA chaperone activities.
转录后修饰在生命的所有三个域的核糖体RNA成熟过程中发挥着许多重要作用。核糖2'-甲基化是最丰富的化学rRNA修饰，例如，参与RNA折叠和稳定。在古细菌中，这些修饰位点由可变的C/D盒sRNA集合决定，这些sRNA指导rRNA 2'-甲基转移酶fibrillarin的活性。每个C/D盒sRNA包含两个指导序列，它们可以协同作用以桥接rRNA序列。在这里，我们将综述古细菌C/D盒sRNA基因及其靶位点的情况。一个重点是指导序列明显加速的进化以及两个单独指导序列的不同配对，这导致了不同的rRNA修饰模式和RNA伴侣活性。
