Department of Clinical Laboratory, Linyi People's Hospital, Linyi, Shandong, China.
Eur Rev Med Pharmacol Sci. 2017 May;21(9):2103-2107.
This study aims to investigate long non-coding RNA LINC01133 (LINC01133) expressions in colorectal cancer (CRC) patients, and discuss its correlation with CRC clinicopathological features and prognosis.
qRT-PCR was performed to measure expression levels of LINC01133 in CRC tissues. The chi-square test was used to assess LINC01133 expression with respect to clinicopathological parameters. Kaplan-Meier analysis and the log-rank test were performed to identify survival differences in CRC patients. Univariate and multivariate analysis were performed using the Cox proportional hazard analysis.
LINC01133 was significantly down-regulated in CRC tissues compared to normal tissue samples (p < 0.001), and a low expression of LINC01133 was found to be significantly associated with lymph node metastasis (p = 0.004), distant metastasis (p = 0.043), N classification (p = 0.022) and TNM stage (p = 0.011). Moreover, Kaplan-Meier survival analysis revealed that high LINC01133 expression predicted significantly better overall survival (p = 0.0093). Finally, multivariate analysis results indicated that LINC01133 was an independent prognostic factor in CRC.
Our results indicated that reduced LINC01133 expression contributed to CRC metastasis and poor prognosis. Thus, LINC01133 might serve as a promising biomarker for prognosis of CRC.
本研究旨在探讨长链非编码 RNA LINC01133（LINC01133）在结直肠癌（CRC）患者中的表达，并探讨其与 CRC 临床病理特征和预后的关系。
采用 qRT-PCR 检测 CRC 组织中 LINC01133 的表达水平。采用卡方检验评估 LINC01133 表达与临床病理参数的关系。采用 Kaplan-Meier 分析和对数秩检验比较 CRC 患者的生存差异。采用单因素和多因素 Cox 比例风险分析进行分析。
与正常组织样本相比，CRC 组织中 LINC01133 表达显著下调（p<0.001），且 LINC01133 低表达与淋巴结转移（p=0.004）、远处转移（p=0.043）、N 分类（p=0.022）和 TNM 分期（p=0.011）显著相关。此外，Kaplan-Meier 生存分析显示，LINC01133 高表达预示着总生存期显著延长（p=0.0093）。最后，多因素分析结果表明，LINC01133 是 CRC 的独立预后因素。
本研究结果表明，LINC01133 表达降低与 CRC 转移和不良预后有关。因此，LINC01133 可能成为 CRC 预后的有前途的生物标志物。
