Scheuermann D W, Stach W, Timmermans J P
Acta Anat (Basel). 1986;127(4):290-5. doi: 10.1159/000146300.
The present scanning electron microscopical (SEM) study was initiated to visualize the surface topography of Auerbach's plexus in the ileum of the pig. After enzymatic digestion of the connective-tissue components of the tunica muscularis and the tunica serosa followed by glutaraldehyde fixation, HCl-hydrolysis and stripping off either the longitudinal or circular smooth muscle layer, the three-dimensional architecture and topographical features of the myenteric plexus can clearly be observed. In this way, ganglia, primary, secondary and tertiary strands, and single nerve fibres can be demonstrated. Individual nerve cells, which are incompletely covered by glial cells and by remnants of the basal lamina, can be recognized in the centre and periphery of the ganglia and adjacent to primary and secondary nerve strands.
开展本次扫描电子显微镜（SEM）研究是为了观察猪回肠中奥尔巴赫神经丛的表面形态。在对肌层和浆膜的结缔组织成分进行酶消化，随后进行戊二醛固定、盐酸水解并剥去纵行或环形平滑肌层后，可以清晰地观察到肌间神经丛的三维结构和形态特征。通过这种方式，可以展示神经节、一级、二级和三级神经束以及单根神经纤维。在神经节的中央和周边以及与一级和二级神经束相邻的位置，可以识别出单个神经细胞，这些细胞并未被神经胶质细胞和基膜残余物完全覆盖。
