Medical Genetic Centre, Guangdong Women and Children's Hospital, Guangzhou Medical University, Guangzhou 511442, People's Republic of China.
Medical Genetic Centre, Guangdong Women and Children's Hospital, Guangzhou Medical University, Guangzhou 511442, People's Republic of China; Medical Genetic Centre, Guangdong Women and Children Hospital, Guangzhou 511442, People's Republic of China.
Infect Genet Evol. 2022 Jul;101:105285. doi: 10.1016/j.meegid.2022.105285. Epub 2022 Apr 18.
Genital Chlamydia trachomatis (CT) is one of the most common agents of sexually transmitted infections and can cause severe disorders. This study aimed to analyse the genetic and clinical characteristics of genital CT infection among women in Guangzhou, China.
From September 2020 to August 2021, a total of 8955 female patients were enrolled in this study. The presence of genital CT was detected by real-time PCR, and 273 positive samples were randomly selected for further genetic and clinical characteristics analysis.
The positive rate of genital CT infection was 7.5% (670/8955), with the highest rate in women aged 21-30 years. A total of 8 genotypes were identified: DH, J, K, and recombinant genotype Ba/D. The predominant genotype was J (n = 78, 28.6%), followed by E (n = 63, 23.1%), F (n = 48, 17.6%), and D (n = 38, 13.9%). Abnormal vaginal discharge (n = 165, 61.8%), cervical columnar epithelial ectopy (n = 124, 46.4%), vaginal itching (n = 77, 28.8%), and lower abdominal pain (n = 61, 22.8%) were the predominant symptoms. Additionally, genotype G infection exhibited a significantly higher rate of abnormal vaginal discharge (P = 0.03) and genotype D infection exhibited a higher white blood cell count (P = 0.01) than the other genotypes. Phylogenetic analysis revealed a total of 20 variants with 25 mutation positions and the H2 variant in four patients was first discovered in our study.
Genotypes J, E, F, and D were the major genotypes of genital CT in Guangzhou, and they manifested as abnormal vaginal discharge, cervical columnar epithelial ectopy, vaginal itching, and lower abdominal pain. The present study provides guidance for future integrated interventions to reduce the burden of genital CT infection and accelerate the development of vaccines.
生殖道沙眼衣原体（CT）是最常见的性传播感染病原体之一，可引起严重疾病。本研究旨在分析中国广州女性生殖道 CT 感染的遗传和临床特征。
2020 年 9 月至 2021 年 8 月，共纳入 8955 例女性患者。采用实时 PCR 检测生殖道 CT 的存在，随机选择 273 例阳性样本进行进一步的遗传和临床特征分析。
生殖道 CT 感染的阳性率为 7.5%（670/8955），以 21-30 岁女性最高。共鉴定出 8 种基因型：DH、J、K 和重组基因型 Ba/D。主要基因型为 J（n=78，28.6%），其次为 E（n=63，23.1%）、F（n=48，17.6%）和 D（n=38，13.9%）。异常阴道分泌物（n=165，61.8%）、宫颈柱状上皮异位（n=124，46.4%）、阴道瘙痒（n=77，28.8%）和下腹痛（n=61，22.8%）是主要症状。此外，基因型 G 感染的异常阴道分泌物发生率显著较高（P=0.03），基因型 D 感染的白细胞计数较高（P=0.01）。系统发育分析显示，共有 20 个变异体，25 个突变位置，其中 4 例患者的 H2 变异体为本研究首次发现。
广州地区生殖道 CT 的主要基因型为 J、E、F 和 D，表现为异常阴道分泌物、宫颈柱状上皮异位、阴道瘙痒和下腹痛。本研究为未来综合干预生殖道 CT 感染、减轻感染负担和加速疫苗开发提供了指导。
