Department of Bio and Nano Technology, Guru Jambheshwar University of Science and Technology, Hisar, Haryana 125001, India.
Department of Bio and Nano Technology, Guru Jambheshwar University of Science and Technology, Hisar, Haryana 125001, India; Department of Mechanical Engineering, UIET, Panjab University, Chandigarh 160014, India.
Int J Food Microbiol. 2022 Jul 2;372:109677. doi: 10.1016/j.ijfoodmicro.2022.109677. Epub 2022 Apr 11.
Worldwide, fungal contamination of water resources has become a major threat to both human health and the environment. The adaptation of nanotechnology in conventional water processes is significant to offer new breakthroughs in water treatment, especially fungal contaminants. Chitosan conjugated metal oxide nanoparticles can affect the antimicrobial properties of cellulosic foam. In the present study, three different types of biocompatible nanoconjugates (i.e., ZnO/chitosan, CuO/chitosan, and AgO/chitosan) were synthesized for functionalization of five differently processed cellulose foam filters for resisting fungal spores during water treatment. To evaluate the antifungal effect of these nanoconjugates against prevalent strains of Aspergillus niger (A. niger), Aspergillus flavus (A. flavus), and Rhizopus oryzae (R. oryzae), the stable coating was introduced on different cellulose filter papers through impregnation. The statistical analysis of antifungal experiment was carried out by two-way factorial ANOVA test. Cellulose filter containing ZnO/chitosan displayed a stronger antifungal behavior in disc diffusion method than those impregnated with CuO/chitosan, and AgO/chitosan nanoconjugates. Besides the choice of nanoconjugates, the variation in cellulose foam filters (in terms of concentration of their raw materials and/or processing methodology) can also affect their antifungal performance. Further, the assessment of cytotoxic nature of such nanocomposites-modified cellulose foam filters is a fundamental step towards their real field applications.
在全球范围内，水资源中的真菌污染已成为人类健康和环境的主要威胁。将纳米技术应用于常规水工艺对于提供水处理的新突破具有重要意义，尤其是真菌污染物。壳聚糖结合金属氧化物纳米粒子可以影响纤维素泡沫的抗菌性能。在本研究中，合成了三种不同类型的生物相容性纳米复合物（即 ZnO/壳聚糖、CuO/壳聚糖和 AgO/壳聚糖），用于对五种不同处理的纤维素泡沫过滤器进行功能化，以抵抗水处理过程中的真菌孢子。为了评估这些纳米复合物对流行的黑曲霉（A. niger）、黄曲霉（A. flavus）和米根霉（R. oryzae）菌株的抗真菌效果，通过浸渍将稳定的涂层引入到不同的纤维素滤纸上。通过双因素方差分析（ANOVA）测试对抗真菌实验进行了统计分析。在圆盘扩散法中，含 ZnO/壳聚糖的纤维素过滤器比浸渍有 CuO/壳聚糖和 AgO/壳聚糖纳米复合物的过滤器表现出更强的抗真菌行为。除了纳米复合物的选择外，纤维素泡沫过滤器（就其原材料的浓度和/或加工方法而言）的变化也会影响其抗真菌性能。此外，评估此类纳米复合材料修饰的纤维素泡沫过滤器的细胞毒性性质是朝着其实际现场应用迈出的基本步骤。
