Javed Rabia, Rais Farwa, Fatima Humaira, Haq Ihsan Ul, Kaleem Muhammad, Naz Syeda Sohaila, Ao Qiang
Department of Tissue Engineering, China Medical University, Shenyang 110122, China; Department of Biological Sciences, National University of Medical Sciences, Rawalpindi 46000, Pakistan.
Department of Dental Materials, Army Medical College, National University of Medical Sciences, Rawalpindi 46000, Pakistan.
Mater Sci Eng C Mater Biol Appl. 2020 Nov;116:111184. doi: 10.1016/j.msec.2020.111184. Epub 2020 Jun 11.
Current report is paramount contribution via nanotechnology to the existing remedies of health diseases. The lag in application of capped metallic oxide nanoparticles in restorative dentistry exist which is covered by this promising study. The uncapped and chitosan encapsulated ZnO nanoparticles were fabricated by facile co-precipitation method, and characterized using various biophysical strategies including X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), Scanning electron microscopy (SEM), Transmission electron microscopy (TEM) and Energy dispersive x-ray (EDX). ZnO nanoparticles and ZnO-Citosan nanoparticles were estimated to be <30 nm and <25 nm in size on respective basis. Significant in vitro antibacterial, antioxidant, cytotoxic and antidiabetic activity of ZnO nanoparticles has been elucidated that is enhanced by capping with chitosan polymer. 90% cytotoxicity against brine shrimps, 69.6% antidiabetic activity against α-amylase, and noteworthy antioxidation power by chitosan decorated ZnO nanoparticles has been effectively illustrated. Furthermore, the effective secondary caries remediation approach has been established by an amalgamation of ZnO nanoparticles and ZnO-Chitosan nanoparticles into dentine bonding agents. A remarkable reduction in Streptococcus mutans and Lactobacillus acidophillus strains has been observed, in-specific boosted by chitosan capped ZnO nanoparticles reinforced dental adhesive discs. Additionally, augmented mechanical properties, greater resistance to water sorption and solubility, notably high release profile, and slight variation of shear bond strength values have been obtained. In short, the prepared nanoparticles reported are detected to be auspicious theranostic agents for combating wide array of human pathogens in healthcare system.
当前报告是纳米技术对现有健康疾病治疗方法的重要贡献。本项前景广阔的研究涵盖了包覆金属氧化物纳米颗粒在修复牙科应用方面的滞后情况。通过简便的共沉淀法制备了未包覆和壳聚糖包覆的氧化锌纳米颗粒，并使用包括X射线衍射（XRD）、傅里叶变换红外光谱（FTIR）、扫描电子显微镜（SEM）、透射电子显微镜（TEM）和能量色散X射线（EDX）在内的各种生物物理策略对其进行了表征。氧化锌纳米颗粒和氧化锌 - 壳聚糖纳米颗粒的尺寸分别估计小于30纳米和小于25纳米。已阐明氧化锌纳米颗粒具有显著的体外抗菌、抗氧化、细胞毒性和抗糖尿病活性，用壳聚糖聚合物包覆可增强这些活性。已有效证明壳聚糖修饰的氧化锌纳米颗粒对卤虫具有90%的细胞毒性、对α - 淀粉酶具有69.6%的抗糖尿病活性以及显著的抗氧化能力。此外，通过将氧化锌纳米颗粒和氧化锌 - 壳聚糖纳米颗粒与牙本质粘结剂混合，建立了有效的继发龋修复方法。已观察到变形链球菌和嗜酸乳杆菌菌株显著减少，特别是壳聚糖包覆的氧化锌纳米颗粒增强的牙科粘结盘对此有明显促进作用。此外，还获得了增强的机械性能、更大的耐吸水性和溶解性、显著较高的释放曲线以及剪切粘结强度值的轻微变化。简而言之，所报道的制备纳米颗粒被检测为在医疗保健系统中对抗多种人类病原体的 auspicious 治疗诊断剂。 （注：“auspicious”此处根据语境不太好准确翻译，暂保留英文）
