Berger S A, Battat A, Halperin E, Stein J, Dan M
Am J Clin Pathol. 1987 Feb;87(2):272-5. doi: 10.1093/ajcp/87.2.272.
Each of 1,018 blood culture specimens was inoculated into Bactec 6B (Johnston Laboratories, Towson, MD) and a biphasic blood culture system that incorporates internal removable agar dip slides (Hylab). Ninety clinically significant pathogens were recovered: 66 from both systems, 14 from Bactec only, and 10 from Hylab only. The mean incubation times to positive of the two systems did not differ when data were examined for gram-negative bacilli, gram-positive cocci, total isolates, and contaminants (P greater than 0.05). Contamination rates were also comparable: Bactec 5.4%, Hylab 7.3% (P greater than 0.05). The Hylab system may offer a practical alternative to Bactec 6B.
1018份血培养标本每份都接种到Bactec 6B(约翰斯顿实验室,马里兰州陶森)和一种包含内部可移除琼脂浸片的双相血培养系统(Hylab)中。共分离出90株具有临床意义的病原体:66株在两种系统中均能分离到,14株仅在Bactec系统中分离到,10株仅在Hylab系统中分离到。当对革兰氏阴性杆菌、革兰氏阳性球菌、总分离株和污染物的数据进行检查时,两种系统阳性的平均孵育时间没有差异(P大于0.05)。污染率也相当:Bactec为5.4%,Hylab为7.3%(P大于0.05)。Hylab系统可能是Bactec 6B的一种实用替代方案。