Herbet Mariola, Piątkowska-Chmiel Iwona, Motylska Monika, Gawrońska-Grzywacz Monika, Nieradko-Iwanicka Barbara, Dudka Jarosław
Department of Toxicology, Faculty of Pharmacy, Medical University of Lublin, Jaczewskiego 8b Street, 20-090 Lublin, Poland.
Department of Hygiene and Epidemiology, Faculty of Medical Sciences, Medical University of Lublin, Chodźki 7 Street, 20-093 Lublin, Poland.
Brain Sci. 2022 Apr 13;12(4):498. doi: 10.3390/brainsci12040498.
Exposure to chronic stress leads to disturbances in glucose metabolism in the brain, and changes in the functioning of neurons coexisting with the development of depression. The detailed molecular mechanism and cerebral gluconeogenesis during depression are not conclusively established. The aim of the research was to assess the expression of selected genes involved in cerebral glucose metabolism of mice in the validated animal paradigm of chronic stress. To confirm the induction of depression-like disorders, we performed three behavioral tests: sucrose preference test (SPT), forced swim test (FST), and tail suspension test (TST). In order to study the cerebral glucose metabolism of the brain, mRNA levels of the following genes were determined in the prefrontal cortex of mice: , , , , and . It has been shown that exogenous, chronic administration of corticosterone developed a model of depression in behavioral tests. There were statistically significant changes in the mRNA level of the , , , and genes. The obtained results suggest changes in cerebral glucose metabolism as a process of adaptation to stressful conditions, and may provide the basis for introducing new therapeutic strategies for chronic stress-related depression.
长期暴露于应激状态会导致大脑葡萄糖代谢紊乱，以及与抑郁症发展并存的神经元功能变化。抑郁症期间详细的分子机制和脑内糖异生尚未最终确定。本研究的目的是在经过验证的慢性应激动物模型中评估参与小鼠脑葡萄糖代谢的特定基因的表达。为了证实类似抑郁症障碍的诱导，我们进行了三项行为测试：蔗糖偏好试验（SPT）、强迫游泳试验（FST）和悬尾试验（TST）。为了研究大脑的脑葡萄糖代谢，测定了小鼠前额叶皮质中以下基因的mRNA水平： 、 、 、 和 。研究表明，外源性长期给予皮质酮在行为测试中建立了抑郁症模型。 、 、 和 基因的mRNA水平存在统计学上的显著变化。所得结果表明，脑葡萄糖代谢变化是对压力条件的一种适应过程，可能为引入针对慢性应激相关抑郁症的新治疗策略提供依据。
