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通过生物相容性 AIE 探针，可视化利用微藻作为可持续生物工厂生产健康脂质的新兴平台。

Visualising the Emerging Platform of Using Microalgae as a Sustainable Bio-Factory for Healthy Lipid Production through Biocompatible AIE Probes.

机构信息

College of Science and Engineering, Flinders University, Adelaide, SA 5001, Australia.

Institute for NanoScale Science and Technology, College of Science and Engineering, Flinders University, Adelaide, SA 5001, Australia.

出版信息

Biosensors (Basel). 2022 Mar 31;12(4):208. doi: 10.3390/bios12040208.

DOI:10.3390/bios12040208
PMID:35448268
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9029145/
Abstract

Nowadays, a particular focus is using microalgae to get high-valued health beneficiary lipids. The precise localisation of the lipid droplets (LDs) and biochemical changes are crucial to portray the lipid production strategy in algae, but it requires an in vivo tool to rapidly visualise LD distribution. As a novel strategy, this study focuses on detecting lipid bioaccumulation in a green microalga, using the aggregation-induced emission (AIE) based probe, 2-DPAN (CHNO). As the messenger molecule and stress biomarker, hydrogen peroxide (HO) activity was detected in lipid synthesis with the AIE probe, TPE-BO (CHBO). Distinctive LDs labelled with 2-DPAN have elucidated the lipid inducing conditions, where more health beneficiary α-linolenic acid has been produced. TPE-BO labelled HO have clarified the involvement of HO during lipid biogenesis. The co-staining procedure with traditional green BODIPY dye and red chlorophyll indicates that 2-DPAN is suitable for multicolour LD imaging. Compared with BODIPY, 2-DPAN was an efficient sample preparation technique without the washing procedure. Thus, 2-DPAN could improve traditional fluorescent probes currently used for lipid imaging. In addition, the rapid, wash-free, multicolour AIE-based in vivo probe in the study of LDs with 2-DPAN could advance the research of lipid production in microalgae.

摘要

如今，人们特别关注利用微藻获取高附加值的有益健康的脂类。准确定位脂滴（LDs）和生化变化对于描述藻类中的脂质生产策略至关重要，但这需要一种体内工具来快速可视化 LD 分布。作为一种新策略，本研究专注于使用基于聚集诱导发射（AIE）的探针 2-DPAN（CHNO）检测绿色微藻中的脂质生物积累。作为信使分子和应激生物标志物，H2O2（HO）活性在脂质合成中用 AIE 探针 TPE-BO（CHBO）进行了检测。用 2-DPAN 标记的独特 LDs 阐明了诱导脂质的条件，在此条件下产生了更多有益健康的α-亚麻酸。TPE-BO 标记的 HO 阐明了 HO 在脂质生物发生过程中的参与。与传统的绿色 BODIPY 染料和红色叶绿素的共染色程序表明，2-DPAN 适用于多色 LD 成像。与 BODIPY 相比，2-DPAN 是一种有效的样品制备技术，无需洗涤步骤。因此，2-DPAN 可以改进目前用于脂质成像的传统荧光探针。此外，本研究中使用 2-DPAN 的快速、免洗、多色基于 AIE 的体内探针在 LDs 研究方面的应用，有望推进微藻中脂质生产的研究。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1eff/9029145/a6b3802edac8/biosensors-12-00208-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1eff/9029145/86599e89fb98/biosensors-12-00208-g001.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1eff/9029145/491b34d12b03/biosensors-12-00208-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1eff/9029145/ad1180f5450a/biosensors-12-00208-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1eff/9029145/0918a1d2242d/biosensors-12-00208-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1eff/9029145/a6b3802edac8/biosensors-12-00208-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1eff/9029145/86599e89fb98/biosensors-12-00208-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1eff/9029145/d5a71a484362/biosensors-12-00208-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1eff/9029145/d57ebc6f3f09/biosensors-12-00208-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1eff/9029145/e0444e74c57b/biosensors-12-00208-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1eff/9029145/491b34d12b03/biosensors-12-00208-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1eff/9029145/ad1180f5450a/biosensors-12-00208-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1eff/9029145/0918a1d2242d/biosensors-12-00208-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1eff/9029145/a6b3802edac8/biosensors-12-00208-g008.jpg

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