College of Science and Engineering, Flinders University, South Australia, 5042, Australia.
Institute for NanoScale Science and Technology, Medical Device Research Institute, College of Science and Engineering, Flinders University, South Australia, 5042, Australia.
Adv Healthc Mater. 2021 Dec;10(24):e2101223. doi: 10.1002/adhm.202101223. Epub 2021 Sep 1.
Reactive oxygen species (ROS) are highly reactive molecules, serve the normal signaling in different cell types. Targeting ROS as the chemical signals, different stress based strategies have been developed to synthesis different anti-inflammatory molecules in microalgae. These molecules could be utilized as health supplements in human. To provoke the ROS-mediated defence systems, their connotation with the associated conditions must be well understood, therefore, proper tools for studying ROS in natural state are essential. The in vivo detection of ROS with phosphorescent probes offers promising opportunities to study these molecules in a non-invasive manner. Most of the common problems in the traditional fluorescent probes are lower photostability, excitation intensity, slow responsiveness, and the microenvironment that challenge their performance. Some ROS-specific aggregationinduced emission luminogens (AIEgens) with pronounced spatial and temporal resolution have recently demonstrated high selectivity, rapid responsiveness, and efficacies to resolve the aggregation-caused quenching issues. The nanocomposites of some AIE-photosensitizers can also improve the ROS-mediated photodynamic therapy. These AIEgens could be used to induce bioactive components in microalgae through altering the ROS signaling, therefore are more auspicious for biomedical research. This study reviews the prospects of AIEgen-based technologies to understand the ROS mediated bio-physiological processes in microalgae for better healthcare benefits.
活性氧（ROS）是高度反应性的分子，在不同的细胞类型中起正常信号作用。将 ROS 作为化学信号靶向，已经开发了基于不同应激的策略来在微藻中合成不同的抗炎分子。这些分子可以用作人类的保健品。为了引发 ROS 介导的防御系统，必须充分了解它们与相关条件的内涵，因此，研究自然状态下 ROS 的适当工具是必不可少的。用磷光探针进行体内 ROS 检测为非侵入性研究这些分子提供了有希望的机会。传统荧光探针中存在的大多数常见问题，如较低的光稳定性、激发强度、较慢的响应性以及微环境，都影响了它们的性能。一些具有显著时空分辨率的 ROS 特异性聚集诱导发射发光团（AIEgens）最近表现出高选择性、快速响应性和功效，可以解决聚集引起的猝灭问题。一些 AIE-光敏剂的纳米复合材料也可以提高 ROS 介导的光动力疗法。这些 AIEgens 可以通过改变 ROS 信号来诱导微藻中的生物活性成分，因此更有利于生物医学研究。本研究综述了基于 AIEgen 的技术的前景，以了解微藻中 ROS 介导的生物生理过程，从而获得更好的医疗保健益处。
