Zhou Zixuan, Chen Yan, Qian Xiang
Tsinghua-Shenzhen International Graduate School, Tsinghua University, Shenzhen 518055, China.
Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China.
Biosensors (Basel). 2022 Apr 18;12(4):257. doi: 10.3390/bios12040257.
Exosomes (30-100 nm in diameter) are a group of cell-derived membrane vesicles, packaged as valuable cargo with lipid, proteins, and genetic materials from their parent cells. With the increasing interest in exosomes for diagnostic and therapeutic applications, the rapid isolation of pure exosome populations has become a hot topic. In this paper, we propose modified microchannels with aptamer in a microfluidics system for rapid and efficient isolation of exosomes by targeting exosome-carrying CD63 and PTK 7. The capture efficiency in surface-modified channels reaches around 10-10 particles/mL in 20 min, and purified exosomes with reliable size can be achieved.
外泌体（直径30 - 100纳米）是一组细胞来源的膜泡，作为有价值的“货物”包裹着来自其母细胞的脂质、蛋白质和遗传物质。随着对外泌体在诊断和治疗应用方面的兴趣日益增加，快速分离纯外泌体群体已成为一个热门话题。在本文中，我们提出在微流控系统中使用带有适配体的改良微通道，通过靶向携带外泌体的CD63和PTK 7来快速高效地分离外泌体。表面修饰通道的捕获效率在20分钟内达到约10 - 10个颗粒/毫升，并且可以获得尺寸可靠的纯化外泌体。
