Ahmed Hanaa Y, Safwat Nesreen, Shehata Reda, Althubaiti Eman Hillal, Kareem Sayed, Atef Ahmed, Qari Sameer H, Aljahani Amani H, Al-Meshal Areej Suliman, Youssef Mahmoud, Sami Rokayya
The Regional Center for Mycology and Biotechnology, Al-Azhar University, Cairo 11787, Egypt.
Department of Biotechnology, Faculty of Science, Taif University, P.O. Box 11099, Taif 21944, Saudi Arabia.
Membranes (Basel). 2022 Apr 8;12(4):408. doi: 10.3390/membranes12040408.
Hydroxyapatite nanoparticles (HAn) have been produced as biomaterial from biowaste, especially snail shells (. It is critical to recycle the waste product in a biomedical application to overcome antibiotic resistance as well as biocompatibility with normal tissues. Moreover, EDX, TEM, and FT-IR analyses have been used to characterize snail shells and HAn. The particle size of HAn is about 15.22 nm. Furthermore, higher inhibitory activity was observed from HAn than the reference compounds against all tested organisms. The synthesized HAn has shown the lowest MIC values of about 7.8, 0.97, 3.9, 0.97, and 25 µg/mL for , , , , and , respectively. In addition, the HAn displayed potent antibiofilm against and . According to the MTT, snail shell and HAn had a minor influence on the viability of HFS-4 cells. Consequently, it could be concluded that some components of waste, such as snail shells, have economic value and can be recycled as a source of CaO to produce HAn, which is a promising candidate material for biomedical applications.
羟基磷灰石纳米颗粒（HAn）已由生物废料，特别是蜗牛壳制备成生物材料。在生物医学应用中回收废品以克服抗生素耐药性以及与正常组织的生物相容性至关重要。此外，能量散射X射线谱（EDX）、透射电子显微镜（TEM）和傅里叶变换红外光谱（FT-IR）分析已用于表征蜗牛壳和HAn。HAn的粒径约为15.22纳米。此外，与参考化合物相比，HAn对所有测试生物体均表现出更高的抑制活性。合成的HAn对金黄色葡萄球菌、大肠杆菌、铜绿假单胞菌、白色念珠菌和热带念珠菌的最低抑菌浓度（MIC）值分别约为7.8、0.97、3.9、0.97和25微克/毫升。此外，HAn对金黄色葡萄球菌和白色念珠菌表现出强大的抗生物膜能力。根据MTT法，蜗牛壳和HAn对人皮肤成纤维细胞系HFS-4细胞的活力影响较小。因此，可以得出结论，一些废品成分，如蜗牛壳，具有经济价值，可作为氧化钙来源回收利用以生产HAn，HAn是生物医学应用中一种很有前景的候选材料。
