Baek Ji-Won, Park Ho, Kim Ki-Su, Chun Sung-Kun, Kim Beom-Su
Department of R&BD, Cellco Inc., 208, Venture Startup Center, Jeonju University, 303, Cheonjam-ro, Wansan-gu, Jeonju-si 55069, Korea.
Department of Clinical Laboratory Science, Wonkwang Health Science University, 514, Iksan-daero, Iksan-si 54538, Korea.
Materials (Basel). 2022 May 10;15(10):3413. doi: 10.3390/ma15103413.
Powder-based 3D printing is an excellent technique for the fabrication of complex structural shapes. The outstanding bone remodeling capacity of calcium phosphate bioceramics is a desirable characteristic for such fabrication. Whitlockite (WH) is a calcium phosphate-based ceramic that contains Mg ions and possesses good mechanical properties, rapid resorbability, and promotes osteogenesis. The aim of this study was to fabricate 3D-printed scaffolds using marine plankton-derived WH (MP-WH) powder. The surface morphology and composition of the fabricated scaffolds were characterized by scanning electron microscopy and X-ray diffraction. The biocompatibility and osteogenic effects were evaluated using human mesenchymal stem cells. We successfully obtained a 3D porous scaffold using MP-WH. The MP-WH 3D scaffold showed improved compressive strength compared to the tricalcium phosphate (TCP) 3D scaffold. The in vitro results showed that compared with TCP 3D scaffolds, MP-WH 3D scaffolds were biocompatible and enhanced cell proliferation and adhesion. In addition, alkaline phosphatase activity and real-time polymerase chain reaction assays demonstrated that osteoblast differentiation was improved on the MP-WH scaffold. These results suggest that marine plankton-derived WH is useful for fabricating 3D-printed scaffolds for bone tissue engineering applications.
基于粉末的3D打印是制造复杂结构形状的一项出色技术。磷酸钙生物陶瓷出色的骨重塑能力是这种制造所需的特性。白磷钙矿（WH）是一种含镁离子的磷酸钙基陶瓷，具有良好的机械性能、快速可吸收性，并能促进骨生成。本研究的目的是使用海洋浮游生物衍生的WH（MP-WH）粉末制造3D打印支架。通过扫描电子显微镜和X射线衍射对制造的支架的表面形态和成分进行了表征。使用人间充质干细胞评估了生物相容性和成骨效果。我们成功地使用MP-WH获得了3D多孔支架。与磷酸三钙（TCP）3D支架相比，MP-WH 3D支架的抗压强度有所提高。体外结果表明，与TCP 3D支架相比，MP-WH 3D支架具有生物相容性，并能增强细胞增殖和黏附。此外，碱性磷酸酶活性和实时聚合酶链反应分析表明，MP-WH支架上的成骨细胞分化得到改善。这些结果表明，海洋浮游生物衍生的WH可用于制造用于骨组织工程应用的3D打印支架。
