Evaluation of the Antifungal Activities of and Its Secondary Metabolites against Phytopathogenic .

is a phytopathogenic fungus that causes devastating losses in strawberries without effective countermeasures. Members of the genus exhibit antimicrobial capability and have been found to have the potential for use as biocontrol agents against . species exhibit two phase variations with a differentiated composition of secondary metabolites designated to each phase. In this study, sp. nov. 0813-124 exhibited phase I (PL1) and phase II (PL2); however, only PL1 displayed distinct inhibition of in the confrontation assay. We identified the bioactive ingredients of sp. nov. 0813-124 to be glidobactin A and cepafungin I, with MIC values lower than 1.5 and 2.0 µg/mL, respectively. Furthermore, we revealed the biosynthetic gene cluster (BGC) of corresponding bioactive molecules through genomics analysis and determined its expression level in PL1 and PL2. The expression of glidobactin BGC in PL1 increased rapidly within 24 h, while PL2 was eventually stimulated after 60 h. In summary, we demonstrated that sp. nov. 0813-124 could potentially be used as a biocontrol agent or part of a natural product repertoire for combating .