棉花（棉属）海藻糖-6-磷酸磷酸酶（TPP）基因家族成员的全基因组鉴定及其在干旱胁迫响应中的作用

Genome-Wide Identification of Cotton ( spp.) Trehalose-6-Phosphate Phosphatase (TPP) Gene Family Members and the Role of in the Response to Drought Stress.

作者信息

Wang Weipeng, Cui Hua, Xiao Xiangfen, Wu Bingjie, Sun Jialiang, Zhang Yaxin, Yang Qiuyue, Zhao Yuping, Liu Guoxiang, Qin Tengfei

机构信息

Key Laboratory of Tobacco Improvement and Biotechnology, Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao 266100, China.

College of Agriculture, Liaocheng University, Liaocheng 252059, China.

出版信息

Plants (Basel). 2022 Apr 15;11(8):1079. doi: 10.3390/plants11081079.

Trehalose-6-phosphate phosphatase (TPP) is a key enzyme involved in trehalose synthesis in higher plants. Previous studies have shown that TPP family genes increase yields without affecting plant growth under drought conditions, but their functions in cotton have not been reported. In this study, 17, 12, 26 and 24 TPP family genes were identified in , , and , respectively. The 79 TPP family genes were divided into three subgroups by phylogenetic analysis. Virus-induced gene silencing (VIGS) of produced TRV:: plants that were more sensitive to drought stress than the control plants, and the relative expression of was decreased, as shown by qRT-PCR. Moreover, we analysed the gene structure, targeted small RNAs, and gene expression patterns of TPP family members and the physicochemical properties of their encoded proteins. Overall, members of the TPP gene family in cotton were systematically identified, and the function of under drought stress conditions was preliminarily verified. These findings provide new information for improving drought resistance for cotton breeding in the future.

海藻糖-6-磷酸磷酸酶（TPP）是高等植物中海藻糖合成过程中的关键酶。先前的研究表明，TPP家族基因在干旱条件下可提高产量且不影响植物生长，但其在棉花中的功能尚未见报道。在本研究中，分别在陆地棉、海岛棉、亚洲棉和草棉中鉴定出17个、12个、26个和24个TPP家族基因。通过系统发育分析，将79个TPP家族基因分为三个亚组。对陆地棉的TPP基因进行病毒诱导基因沉默（VIGS），获得了TRV::TPP植株，这些植株比对照植株对干旱胁迫更敏感，并且通过qRT-PCR显示，TPP的相对表达量降低。此外，我们分析了TPP家族成员的基因结构、靶向小RNA、基因表达模式及其编码蛋白的理化性质。总体而言，对棉花TPP基因家族成员进行了系统鉴定，并初步验证了陆地棉TPP基因在干旱胁迫条件下的功能。这些研究结果为未来棉花育种提高抗旱性提供了新信息。