Liu Yue, Wang Xin, Ouyang Lei, Yao Ruonan, Wang Zhihui, Kang Yanping, Yan Liying, Chen Yuning, Huai Dongxin, Wang Qianqian, Jiang Huifang, Lei Yong, Liao Boshou
Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture and Rural Affairs, Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan 430062, China.
State Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei University, Wuhan 430062, China.
Plants (Basel). 2024 Apr 9;13(8):1056. doi: 10.3390/plants13081056.
Trehalose-6-phosphate phosphatase (TPP) is a pivotal enzyme in trehalose biosynthesis which plays an essential role in plant development and in the abiotic stress response. However, little is currently known about in groundnut. In the present study, a total of 16 genes were identified, and can be divided into three phylogenetic subgroups. AhTPP members within the same subgroups generally displayed similar exon-intron structures and conserved motifs. Gene collinearity analysis revealed that segmental duplication was the primary factor driving the expansion of the family. An analysis of the upstream promoter region of revealed eight hormone- and four stress-related responsive -elements. Transcriptomic analysis indicated high expression levels of genes in roots or flowers, while RT-qPCR analysis showed upregulation of the six tested genes under different abiotic stresses, suggesting that play roles in growth, development, and response to various abiotic stresses. Subcellular localization analysis showed that AhTPP1A and AhTPP5A were likely located in both the cytoplasm and the nucleus. To further confirm their functions, the genes and were individually integrated into yeast expression vectors. Subsequent experiments demonstrated that yeast cells overexpressing these genes displayed increased tolerance to osmotic and salt stress compared to the control group. This study will not only lay the foundation for further study of gene functions, but will also provide valuable gene resources for improving abiotic stress tolerance in groundnut and other crops.
海藻糖-6-磷酸磷酸酶（TPP）是海藻糖生物合成中的关键酶，在植物发育和非生物胁迫响应中起重要作用。然而，目前对花生中的该酶了解甚少。在本研究中，共鉴定出16个基因，可分为三个系统发育亚组。同一亚组内的AhTPP成员通常具有相似的外显子-内含子结构和保守基序。基因共线性分析表明，片段重复是驱动该家族扩张的主要因素。对其上游启动子区域的分析揭示了八个激素相关和四个胁迫相关的响应元件。转录组分析表明，该基因在根或花中表达水平较高，而RT-qPCR分析表明，六个被测基因在不同非生物胁迫下上调，表明其在生长、发育以及对各种非生物胁迫的响应中发挥作用。亚细胞定位分析表明，AhTPP1A和AhTPP5A可能定位于细胞质和细胞核。为进一步确认其功能，将这些基因分别整合到酵母表达载体中。随后的实验表明，与对照组相比，过表达这些基因的酵母细胞对渗透胁迫和盐胁迫的耐受性增强。本研究不仅将为进一步研究该基因的功能奠定基础，还将为提高花生和其他作物的非生物胁迫耐受性提供有价值的基因资源。
