Pieracci Ylenia, Vento Martina, Pistelli Luisa, Lombardi Tiziana, Pistelli Laura
Dipartimento di Farmacia, Università di Pisa, Via Bonanno 6, 56126 Pisa, Italy.
Centro Interdipartimentale di Ricerca Nutraceutica e Alimentazione per la Salute 'NUTRAFOOD', Università di Pisa, Via del Borghetto 80, 56124 Pisa, Italy.
Plants (Basel). 2022 Apr 15;11(8):1081. doi: 10.3390/plants11081081.
Halophyte plants are potential resources to deal with the increasing soil salinity determined by climatic change. In this context, the present study aimed to investigate the germplasm conservation of collected in the San Rossore Estate (Pisa, Italy) through in vitro culture, biochemical properties, and the phytochemical composition of the volatile fraction of both in vitro shoots and different organs of wild plants (leaves, young and ripe inflorescences). The best medium tested for the shoot proliferation was MS, with the addition of 1 μM BA. Total polyphenol content and antioxidant activity were noticeable in both the inflorescences, while leaves and in vitro shoots showed lower amounts. Concerning the phytochemical investigation, the headspaces (HSs) and the essential oils (EOs) were characterized by oxygenated monoterpenes as the main chemical class of compounds in all samples, and with α- and β-thujone as the major constituents. However, the EOs were characterized by noticeable percentages of phenylpropanoids (23.6-28.8%), with brevifolin as the unique compound, which was not detected in the spontaneous volatile emissions of the same parts of the wild plant. Good amounts of EOs were obtained from different organs of the wild plant, comprising between 0.17% and 0.41% of the young and ripe inflorescences, respectively.
盐生植物是应对气候变化导致土壤盐度不断增加的潜在资源。在此背景下，本研究旨在通过体外培养、生化特性以及野生植物不同器官（叶片、幼嫩和成熟花序）和体外芽挥发部分的植物化学成分，对在圣罗索尔庄园（意大利比萨）收集的种质进行保存研究。用于芽增殖的最佳培养基是添加了1 μM BA的MS培养基。在花序中总多酚含量和抗氧化活性显著，而叶片和体外芽中的含量较低。关于植物化学研究，顶空（HSs）和精油（EOs）的特征是氧化单萜类化合物是所有样品中主要的化合物类别，α-和β-侧柏酮是主要成分。然而，精油的特征是苯丙素类化合物的含量显著（23.6 - 28.8%），其中短叶苏木酚是唯一的化合物，在野生植物相同部位的自然挥发物中未检测到。从野生植物的不同器官中获得了大量的精油，幼嫩和成熟花序中分别占0.17%至0.41%。
