Department of Biochemistry and Molecular Genetics, Israel Institute for Biological Research, Ness Ziona, Israel.
BMC Genom Data. 2022 Apr 21;23(1):31. doi: 10.1186/s12863-022-01049-7.
As part of a research aiming at the isolation of bacteria secreting growth inhibiting compounds, cultures of Francisella tularensis were implanted in environmental samples and monitored for inhibition zones on agar. Two antibiotic-like secreting bacteria were isolated, their genomic sequence was deciphered and taxonomic profiling analysis classified them as belonging to the Pantoea genus.
Two bacterial isolates exhibiting growth inhibition zones to F. tularensis (LVS) were analyzed using the Oxford Nanopore Technology (ONT). Preliminary de novo assembly of the reads was performed, followed by taxonomic profiling based on Multi Locus Sequence Analysis (MLSA) and implementation of the Average Nucleotide Identity (ANI) measure. The genomic sequences resulted in the identification of two different Pantoea species, denoted EnvD and EnvH. Subsequent de novo genome assembly generated 5 and 10 contigs for EnvD and EnvH, respectively. The largest contig (4,008,183 bps and 3,740,753 bps for EnvD and EnvH, respectively), overlaps to a major extent to the chromosome of closely related Pantoea species. ANI values calculated for both isolates revealed two apparently new species of the Pantoea genus. Our study deciphered the identity of two bacteria producing antibiotic-like compounds, and the genomic sequence revealed they represent distinct Pantoea species.
作为分离分泌生长抑制化合物的细菌的研究的一部分，将土拉弗朗西斯菌的培养物植入环境样本中，并在琼脂上监测抑制区。分离出两种具有抗生素样分泌作用的细菌，对其基因组序列进行解码，并通过分类分析将其归类为泛菌属。
使用牛津纳米孔技术（ONT）分析了两种表现出对土拉弗朗西斯菌（LVS）生长抑制区的细菌分离株。对reads 进行初步从头组装，然后基于多位点序列分析（MLSA）进行分类分析，并实施平均核苷酸同一性（ANI）测量。基因组序列确定了两种不同的泛菌物种，分别表示为 EnvD 和 EnvH。随后对 EnvD 和 EnvH 进行了从头基因组组装，分别产生了 5 个和 10 个 contigs。最大的 contig（分别为 4,008,183 bps 和 3,740,753 bps）在很大程度上与密切相关的泛菌物种的染色体重叠。为这两种分离株计算的 ANI 值揭示了泛菌属的两个明显的新物种。我们的研究阐明了两种产生抗生素样化合物的细菌的身份，基因组序列表明它们代表不同的泛菌物种。
