Institute for Food Safety and Hygiene, Vetsuisse Faculty University of Zurich, Winterthurerstrasse 272, 8057 Zurich, Switzerland.
BCCM/LMG Bacteria Collection, Laboratorium voor Microbiologie, Universiteit Gent, K.L. Ledeganckstraat 35, 9000 Gent, Belgium.
Int J Syst Evol Microbiol. 2010 Dec;60(Pt 12):2786-2792. doi: 10.1099/ijs.0.019430-0. Epub 2010 Jan 8.
Five Gram-negative, facultatively anaerobic, non-spore-forming, coccoid rod-shaped bacterial isolates were obtained from infant formula and an infant formula production environment and were investigated by use of a polyphasic taxonomic study. Biochemical tests and partial rpoB gene sequence analysis of the five isolates revealed that they formed two distinct groups in the family Enterobacteriaceae, closely related to several species of the genera Pantoea and Erwinia, which indicated a phylogenetic position within the genus Pantoea or the genus Erwinia. Multilocus sequence analysis of concatenated partial atpD, gyrB, infB and rpoB gene sequences of two of the isolates suggested that they represented two novel species of the genus Pantoea, phylogenetically related most closely to Pantoea septica. The five isolates had general characteristics consistent with those of the genus Pantoea, and DNA-DNA hybridizations between two representatives and the type strains of their phylogenetically closest relatives based on comparative 16S rRNA gene sequence analysis showed that the isolates represented two novel genospecies. These two genospecies could be differentiated from each other based on fermentation of galacturonate, sorbitol and potassium 5-ketogluconate. They could be differentiated from phylogenetically related Pantoea species based on their ability to ferment lactose and to utilize β-gentiobiose and raffinose, their inability to ferment or utilize d-arabitol, and their inability to produce indole. On the basis of the results obtained, the five isolates are considered to represent two novel species of the genus Pantoea, for which the names Pantoea gaviniae sp. nov. (type strain A18/07(T) =LMG 25382(T) =DSM 22758(T)) and Pantoea calida sp. nov. (type strain 1400/07(T) =LMG 25383(T) =DSM 22759(T)) are proposed.
从婴儿配方奶粉和婴儿配方奶粉生产环境中获得了五株革兰氏阴性、兼性厌氧、非芽孢形成、球形杆状细菌分离株，并通过多相分类学研究进行了研究。对这五株分离株的生化试验和部分 rpoB 基因序列分析表明，它们在肠杆菌科形成了两个不同的群体，与泛菌属和欧文氏菌属的几个种密切相关，这表明它们在泛菌属或欧文氏菌属内具有系统发育地位。对其中两个分离株的连接部分 atpD、gyrB、infB 和 rpoB 基因序列的多位序列分析表明，它们代表了泛菌属的两个新种，与粪产碱菌亲缘关系最密切。这五个分离株具有与泛菌属一般特征一致的特征，并且基于比较 16S rRNA 基因序列分析的两个代表菌株和它们亲缘关系最密切的模式菌株之间的 DNA-DNA 杂交表明，这些分离株代表了两个新的种。这两个种可以根据对半乳糖醛酸盐、山梨糖醇和钾 5-酮葡萄糖酸盐的发酵来区分。它们可以根据它们发酵乳糖和利用β-龙胆二糖和棉子糖的能力以及它们不能发酵或利用 D-阿拉伯糖醇以及不能产生吲哚来与亲缘关系较近的泛菌属物种区分开来。基于获得的结果，这五个分离株被认为代表了泛菌属的两个新种，它们的名称分别为泛菌属加文氏菌（模式菌株 A18/07(T)=LMG 25382(T)=DSM 22758(T)）和泛菌属温暖菌（模式菌株 1400/07(T)=LMG 25383(T)=DSM 22759(T)）。
