Department of Smart Green Technology Engineering, Pukyong National University, Busan 48513, South Korea.
Department of Display Engineering, Pukyong National University, Busan 48513, South Korea.
Carbohydr Polym. 2022 Jul 15;288:119389. doi: 10.1016/j.carbpol.2022.119389. Epub 2022 Mar 23.
A novel physically and chemically double-crosslinked hydrogel derived from chitosan oligosaccharide/alginate (COS/Alg) was developed by using norbornene (Nb)-tetrazine (Tz) click reaction for ketoprofen delivery. The properties of the hydrogel were evaluated by rheological, FTIR, TGA, XRD, SEM, swelling and drug release studies. The Nb-Tz chemical cross-linking facilitated outstanding hydrophobic drug loading (44% wt/wt of ketoprofen) and sustained release through a hydrophobic interaction mechanism between the drug and the used polysaccharides. The COS/Alg electrostatics network (10/10 of NH/COOH molar ratio) generated the pH responsiveness, suppressing the release in simulated gastric fluid (below 10% for 2 h) and enhancing the release in simulated intestinal fluids (up to 84% for 24 h). The prepared hydrogel was non-toxic to human HEK-293 cells (95% cell viability). This work opens up a potential approach for preparing hydrophilic hydrogels from natural polysaccharides that can be used in the delivery of hydrophobic drugs.
一种新型的壳聚糖寡糖/海藻酸钠（COS/Alg）物理化学双重交联水凝胶，通过使用降冰片烯（Nb）-四嗪（Tz）点击反应来递送酮洛芬。通过流变学、FTIR、TGA、XRD、SEM、溶胀和药物释放研究来评估水凝胶的性能。Nb-Tz 化学交联有助于出色的疏水性药物负载（酮洛芬的 44%wt/wt）和通过药物与所用多糖之间的疏水相互作用机制实现持续释放。COS/Alg 静电网络（NH/COOH 摩尔比为 10/10）产生 pH 响应性，抑制在模拟胃液中的释放（2 小时内低于 10%），并增强在模拟肠液中的释放（24 小时内高达 84%）。所制备的水凝胶对人 HEK-293 细胞无毒（细胞活力为 95%）。这项工作为从天然多糖制备亲水性水凝胶开辟了一种潜在的方法，可用于疏水性药物的递送。
