Panahandeh Faezeh, Feizi Farideh, Pourghasem Mohsen, Khafri Sorya, Abedian Zeinab, Pourghasem Kaveh, Esmaeili Zohre
Department of Anatomy, Embryology and Histology, Faculty of Medicine, Babol University of Medical Sciences, Babol, Iran.
Department of Anatomy, Embryology and Histology, Faculty of Medicine, Babol University of Medical Sciences, Babol, Iran. Email:
Cell J. 2022 Mar;24(3):148-154. doi: 10.22074/cellj.2022.8054.
Hypothyroidism is known as the most common endocrine disorder. The prevalence of hypothyroidism in the female and male population is 2% and 0.2%, respectively. Maternal hypothyroidism is a defect in the thyroid hormones transition from the mother to the fetus. The present study was conducted to find whether maternal hypothyroidism affects the fertility of the second generation.
In this experimental study, twelve adult female rats weighting 180-220 g were randomly divided into case and control groups. Hypothyroidism was induced by dissolving 0.1 g/L of 6-n-propyl-2-thiouracil in drinking water toward the end of pregnancy and lactation. At the end of the breastfeeding period, the blood samples of female children were collected. Six healthy, mature, female rats were selected and kept until they reached maturity, and were then mated with male rats. After observing the female rats' delivery, blood samples were collected from their male and female newborns and the healthy rats were selected.
There was a significant difference in the volume and size of ovarian as well as in the number of secondary follicles in comparison with the control group (P=0.025). However, there were no significant changes in the other parameters including the number of primary follicles, the number of Graafian follicles and sperm parameters. There was no significant decrease in the testicular volume and size, number of Leydig cells and seminiferous tubules diameter.
Maternal hypothyroidism has no significant effects on testicular tissue function, and sperm parameters in the second generation, but can significantly reduce the rate of secondary follicles in the second generation female rats.
甲状腺功能减退症是最常见的内分泌疾病。女性和男性人群中甲状腺功能减退症的患病率分别为2%和0.2%。母体甲状腺功能减退症是甲状腺激素从母亲向胎儿转移的缺陷。本研究旨在探讨母体甲状腺功能减退症是否会影响第二代的生育能力。
在本实验研究中，将12只体重180 - 220克的成年雌性大鼠随机分为病例组和对照组。在妊娠后期和哺乳期，通过将0.1克/升的6 - 正丙基 - 2 - 硫氧嘧啶溶解于饮用水中来诱导甲状腺功能减退。在哺乳期结束时，采集雌性子代的血样。选取6只健康、成熟的雌性大鼠饲养至成熟，然后与雄性大鼠交配。观察雌性大鼠分娩后，从其雄性和雌性新生子代中采集血样，并选取健康大鼠。
与对照组相比，卵巢的体积和大小以及次级卵泡的数量存在显著差异（P = 0.025）。然而，包括初级卵泡数量、格拉夫卵泡数量和精子参数在内的其他参数没有显著变化。睾丸体积和大小、睾丸间质细胞数量和生精小管直径没有显著减少。
母体甲状腺功能减退症对第二代睾丸组织功能和精子参数没有显著影响，但可显著降低第二代雌性大鼠的次级卵泡率。
