Asada Ryoko, Watanabe Takeru, Tanaka Yoshiharu, Kishida Masao, Furuta Masakazu
Department of Quantum and Radiation Technology, Graduate School of Engineering, Osaka Prefecture University, 1-2, Gakuen-cho, Naka-ku, Sakai, Osaka, 599-8570, Japan.
Department of Biological Science, Graduate School of Science, Osaka Prefecture University, 1-2, Gakuen-cho, Naka-ku, Sakai, Osaka, 599-8570, Japan.
Arch Microbiol. 2022 Apr 22;204(5):275. doi: 10.1007/s00203-022-02892-z.
In this study, we examined the accumulation of trehalose, a stress-responsive substance, upon gamma-ray irradiation by evaluating the cause of trehalose accumulation and the development of gamma-ray resistance through intracellular trehalose accumulation. Saccharomyces cerevisiae cells cultured to the logarithmic growth phase were irradiated with gamma rays, and the intracellular trehalose content was measured. However, trehalose was not detectable. The yeast cells with trehalose accumulation caused by pre-treatment at 40 °C were irradiated with gamma rays, and the resistance of these cells to gamma radiation was compared with that of cells without heat treatment. Trehalose accumulation resulted in gamma-ray resistance and suppressed the increase in reactive oxygen species, lipid peroxidation, and DNA double-strand break production in yeast cells. The tests were also performed with a trehalose-6-phosphate-synthase (TPS1)-deficient mutant strain (Δtps1) unable to synthesize trehalose, and the results revealed that TPS1 was involved in protection against oxidative stress.
在本研究中，我们通过评估海藻糖积累的原因以及通过细胞内海藻糖积累产生γ射线抗性，研究了应激反应物质海藻糖在γ射线照射后的积累情况。将培养至对数生长期的酿酒酵母细胞用γ射线照射，并测量细胞内海藻糖含量。然而，未检测到海藻糖。对经40°C预处理导致海藻糖积累的酵母细胞进行γ射线照射，并将这些细胞对γ射线的抗性与未热处理的细胞进行比较。海藻糖积累导致γ射线抗性，并抑制了酵母细胞中活性氧的增加、脂质过氧化和DNA双链断裂的产生。还用无法合成海藻糖的海藻糖-6-磷酸合酶（TPS1）缺陷突变株（Δtps1）进行了测试，结果表明TPS1参与了抗氧化应激保护。
